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Tumor Biology
Published in: Tumor Biology 11/2016

Open Access 01-11-2016 | Original Article

Integrin-linked kinase regulates cadherin switch in bladder cancer

Authors: Dorota Gil, Dorota Ciołczyk-Wierzbicka, Joanna Dulińska-Litewka, Piotr Laidler

Published in: Tumor Biology | Issue 11/2016

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Abstract

Cadherin switch is specific of epithelial-mesenchymal transition (EMT) and is closely related to tumor cell invasion. However, the molecular mechanism that promotes the phenotypic changes remains unclear and elusive. We found that integrin-linked kinase (ILK) is a key factor involved in cadherin switch. The expression and activity of ILK are elevated in a variety of cancers but its mechanisms are not exactly understood. In this report, we studied the role and mechanism of ILK in EMT of human bladder cancer. We showed that silencing of ILK expression by small interfering RNA (siRNA) significantly abolished the nuclear translocation or the presence of markers associated with EMT like Snail, Twist, Zeb, and beta-catenin. ILK knockdown by siRNA suppressed N-cadherin expression and increased re-expression of E-cadherin in bladder cancer cells. We suggest that ILK is a major signaling factor involved in EMT. It is essential to understand the molecular mechanism of EMT in aim to possibly use it in search for new therapeutic targets.
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Metadata
Title
Integrin-linked kinase regulates cadherin switch in bladder cancer
Authors
Dorota Gil
Dorota Ciołczyk-Wierzbicka
Joanna Dulińska-Litewka
Piotr Laidler
Publication date
01-11-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 11/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-016-5354-x

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