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Tumor Biology
Published in: Tumor Biology 9/2016

01-09-2016 | Original Article

Exosomes derived from gastric cancer cells activate NF-κB pathway in macrophages to promote cancer progression

Authors: Lijun Wu, Xu Zhang, Bin Zhang, Hui Shi, Xiao Yuan, Yaoxiang Sun, Zhaoji Pan, Hui Qian, Wenrong Xu

Published in: Tumor Biology | Issue 9/2016

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Abstract

Exosomes are nano-sized membrane vesicles secreted by both normal and cancer cells. Emerging evidence indicates that cancer cells derived exosomes contribute to cancer progression through the modulation of tumor microenvironment. However, the effects of exosomes derived from gastric cancer cells on macrophages are not well understood. In this study, we investigated the biological role of gastric cancer cells derived exosomes in the activation of macrophages. We demonstrated that gastric cancer cells derived exosomes activated macrophages to express increased levels of proinflammatory factors, which in turn promoted tumor cell proliferation and migration. In addition, gastric cancer cells derived exosomes remarkably upregulated the phosphorylation of NF-κB in macrophages. Inhibiting the activation of NF-κB reversed the upregulation of proinflammatory factors in macrophages and blocked their promoting effects on gastric cancer cells. Moreover, we found that gastric cancer cells derived exosomes could also activate macrophages from human peripheral blood monocytes through the activation of NF-κB. In conclusion, our results suggest that gastric cancer cells derived exosomes stimulate the activation of NF-κB pathway in macrophages to promote cancer progression, which provides a potential therapeutic approach for gastric cancer by interfering with the interaction between exosomes and macrophages in tumor microenvironment.
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Metadata
Title
Exosomes derived from gastric cancer cells activate NF-κB pathway in macrophages to promote cancer progression
Authors
Lijun Wu
Xu Zhang
Bin Zhang
Hui Shi
Xiao Yuan
Yaoxiang Sun
Zhaoji Pan
Hui Qian
Wenrong Xu
Publication date
01-09-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 9/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-016-5071-5

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