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01-06-2016 | Original Article

Lin28A enhances chemosensitivity of colon cancer cells to 5-FU by promoting apoptosis in a let-7 independent manner

Authors: Tianzhen Wang, Peng Han, Yan He, Ci Zhao, Guangyu Wang, Weiwei Yang, Ming Shan, Yuanyuan Zhu, Chao Yang, Mingjiao Weng, Di Wu, Lin Gao, Xiaoming Jin, Yunwei Wei, BinBin Cui, Guomin Shen, Xiaobo Li

Published in: Tumor Biology | Issue 6/2016

Abstract

RNA-binding protein Lin28A is frequently over-expressed in human malignant tumors and is associated with tumor advance and poor prognosis. However, the expression pattern and functions of Lin28A in colon cancer are unknown. In this study, we detected the expression of Lin28A in colon cancer patients and tested the effect of Lin28A on the chemotherapeutic sensitivity of colon cancer cells to 5-fluorouracil (5-FU). As expected, we showed that Lin28A is up-regulated in 73.3 % of colon cancer patients. However, to our surprise, we found that oncogenic protein Lin28A-enforced expression in colon cancer cells enhanced the chemosensitivity of cancer cells to 5-FU via promoting the cell apoptosis. Further mechanisms study revealed that the effect of Lin28A increasing chemosensitivity of cancer cells is in a let-7 independent manner, but which is associated with decreasing the expression of DNA damage repair protein H2AX. Conclusively, here we reported an unexpected function of Lin28A, which may shed lights on fully understanding the physiological and pathological roles of this oncogene.

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Title: Lin28A enhances chemosensitivity of colon cancer cells to 5-FU by promoting apoptosis in a let-7 independent manner
Authors: Tianzhen Wang
Peng Han
Yan He
Ci Zhao
Guangyu Wang
Weiwei Yang
Ming Shan
Yuanyuan Zhu
Chao Yang
Mingjiao Weng
Di Wu
Lin Gao
Xiaoming Jin
Yunwei Wei
BinBin Cui
Guomin Shen
Xiaobo Li
Publication date: 01-06-2016
Publisher: Springer Netherlands
Published in: Tumor Biology / Issue 6/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-015-4559-8

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