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Tumor Biology
Published in: Tumor Biology 12/2015

01-12-2015 | Research Article

Downregulation of FOXP2 promoter human hepatocellular carcinoma cell invasion

Authors: Xia Yan, Huiling Zhou, Tingting Zhang, Pan Xu, Shusen Zhang, Wei Huang, Linlin Yang, Xingxing Gu, Runzhou Ni, Tianyi Zhang

Published in: Tumor Biology | Issue 12/2015

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Abstract

Hepatocellular carcinoma (HCC) is a major health concern with a high morbidity and mortality rate worldwide. However, the mechanism underlying hepatocarcinogenesis remains unclear. Forkhead box P2 (FOXP2) has been implicated in various human cancer types. However, the role of FOXP2 in HCC remains unknown. Western blot and immunohistochemistry were used to measure the expression of FOXP2 protein in HCC and adjacent normal tissues in 50 patients. Wound healing and transwell assays were used to determine the cell invasion ability. We showed that the level of FOXP2 was significantly reduced in HCC compared with the adjacent non-tumorous tissue. There was statistical significance between the expression of FOXP2 and vein invasion (P = 0.017), number of tumor nodes (P = 0.028), and AFP (P = 0.033). Low expression of FOXP2 correlated with poor survival. Moreover, wound healing and transwell assays showed that FOXP2 could decrease cell invasion and affect the expression of vimentin and E-cadherin. Our results suggested that FOXP2 expression was downregulated in HCC tumor tissues, and reduced FOXP2 expression was associated with poor overall survival. In addition, downregulation of FOXP2 significantly enhanced cell invasiveness. These findings uncover that FOXP2 might be a new prognostic factor and be closely correlated with HCC cell invasion.
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Metadata
Title
Downregulation of FOXP2 promoter human hepatocellular carcinoma cell invasion
Authors
Xia Yan
Huiling Zhou
Tingting Zhang
Pan Xu
Shusen Zhang
Wei Huang
Linlin Yang
Xingxing Gu
Runzhou Ni
Tianyi Zhang
Publication date
01-12-2015
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 12/2015
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-3701-y

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