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Tumor Biology
Published in: Tumor Biology 4/2015

01-04-2015 | Research Article

Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway

Authors: Fubin Zhang, Xiaowei Song, Li Li, Jingfang Wang, Leyuan Lin, Cong Li, Hongtao Li, Yanju Lv, Yinghua Jin, Ying Liu, Yu Hu, Tao Xin

Published in: Tumor Biology | Issue 4/2015

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Abstract

One purified polysaccharide protein tyrosine phosphatase (PTP) was isolated from the roots of Polygala tenuifolia. The aim of the present study is to investigate the antitumor effect of PTP on human ovarian cancer OVCAR-3 cells and explore the molecular mechanism of the action involved. The results of MTT assay and apoptosis detection assay showed that PTP inhibited cellular proliferation of OVCAR-3 cells and induced apoptotic cellular death via arresting cell circle at the G0/G1 phase. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis identified that bcl-2 gradually decreased at both transcription and protein levels after PTP treatment for 48 h in OVCAR-3 cells, while those of bax, cytochrome c, caspase-3, and caspase-9 increased. In addition, the low expression of NF-κB in PTP-treated OVCAR-3 cells would trigger the extrinsic pathway of programmed cell death signaling in tumor cells. These results together suggest that PTP may induce apoptosis of OVCAR-3 cells through a mitochondrial pathway.
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Metadata
Title
Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway
Authors
Fubin Zhang
Xiaowei Song
Li Li
Jingfang Wang
Leyuan Lin
Cong Li
Hongtao Li
Yanju Lv
Yinghua Jin
Ying Liu
Yu Hu
Tao Xin
Publication date
01-04-2015
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 4/2015
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-014-2921-x

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