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Tumor Biology
Published in: Tumor Biology 3/2014

01-03-2014 | Research Article

Induction of apoptosis by total flavonoids from Scutellaria barbata D. Don in human hepatocarcinoma MHCC97-H cells via the mitochondrial pathway

Authors: Jie Gao, Wang-Feng Lu, Zhi-Jun Dai, Shuai Lin, Yang Zhao, Sha Li, Nuan-Nuan Zhao, Xi-Jing Wang, Hua-Feng Kang, Xiao-Bin Ma, Wang-Gang Zhang

Published in: Tumor Biology | Issue 3/2014

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Abstract

Scutellaria barbata D. Don, a traditional Chinese medicine, reportedly possesses antitumor activity against a variety of tumors. In the present study, we investigated the cytotoxic effect of total flavonoids from S. barbata (TF-SB) on human hepatocarcinoma cells and the underlying molecular mechanisms regarding the effect were explored. TF-SB treatment significantly reduced the cell viability of human HCC MHCC97-H cells in a dose-dependent manner. Further flow cytometric analysis showed that the apoptosis rate of MHCC97-H cells increased and the mitochondrial membrane potential (∆ψm) of MHCC97-H cells decreased after TF-SB treatment. DNA ladder showed that TF-SB induced a significant increase in DNA fragmentation in MHCC97-H cells. Reverse transcription PCR and Western blot analysis revealed that the expression levels of Smac, Apaf-1, Cytochrome c, Caspase-9, and Caspase-3 were upregulated in a dose-dependent manner and after treatment with different concentrations of TF-SB for 48 h. These results suggest that TF-SB induces apoptosis in MHCC97-H cells through the mitochondrial pathway.
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Metadata
Title
Induction of apoptosis by total flavonoids from Scutellaria barbata D. Don in human hepatocarcinoma MHCC97-H cells via the mitochondrial pathway
Authors
Jie Gao
Wang-Feng Lu
Zhi-Jun Dai
Shuai Lin
Yang Zhao
Sha Li
Nuan-Nuan Zhao
Xi-Jing Wang
Hua-Feng Kang
Xiao-Bin Ma
Wang-Gang Zhang
Publication date
01-03-2014
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 3/2014
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-013-1336-4

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