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01-10-2017 | Original Article

Stroke Induces Mesenchymal Stem Cell Migration to Infarcted Brain Areas Via CXCR4 and C-Met Signaling

Authors: Oh Young Bang, Gyeong Joon Moon, Dong Hee Kim, Ji Hyun Lee, Sooyoon Kim, Jeong Pyo Son, Yeon Hee Cho, Won Hyuk Chang, Yun-Hee Kim, the STARTING-2 trial investigators

Published in: Translational Stroke Research | Issue 5/2017

Abstract

Mesenchymal stem cells circulate between organs to repair and maintain tissues. Mesenchymal stem cells cultured with fetal bovine serum have therapeutic effects when intravenously administered after stroke. However, only a small number of mesenchymal stem cells reach the brain. We hypothesized that the serum from stroke patients increases mesenchymal stem cells trophism toward the infarcted brain area. Mesenchymal stem cells were grown in fetal bovine serum, normal serum from normal rats, or stroke serum from ischemic stroke rats. Compared to the fetal bovine serum group, the stroke serum group but not the normal serum group showed significantly greater migration toward the infarcted brain area in the in vitro and in vivo models (p < 0.05). Both C-X-C chemokine receptor type 4 and c-Met expression levels significantly increased in the stroke serum group than the others. The enhanced mesenchymal stem cells migration of the stroke serum group was abolished by inhibition of signaling. Serum levels of chemokines, cytokines, matrix metalloproteinase, and growth factors were higher in stroke serum than in normal serum. Behavioral tests showed a significant improvement in the recovery after stroke in the stroke serum group than the others. Stroke induces mesenchymal stem cells migration to the infarcted brain area via C-X-C chemokine receptor type 4 and c-Met signaling. Culture expansion using the serum from stroke patients could constitute a novel preconditioning method to enhance the therapeutic efficiency of mesenchymal stem cells.

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Title: Stroke Induces Mesenchymal Stem Cell Migration to Infarcted Brain Areas Via CXCR4 and C-Met Signaling
Authors: Oh Young Bang
Gyeong Joon Moon
Dong Hee Kim
Ji Hyun Lee
Sooyoon Kim
Jeong Pyo Son
Yeon Hee Cho
Won Hyuk Chang
Yun-Hee Kim
the STARTING-2 trial investigators
Publication date: 01-10-2017
Publisher: Springer US
Published in: Translational Stroke Research / Issue 5/2017
Print ISSN: 1868-4483
Electronic ISSN: 1868-601X
DOI: https://doi.org/10.1007/s12975-017-0538-2

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Stroke Induces Mesenchymal Stem Cell Migration to Infarcted Brain Areas Via CXCR4 and C-Met Signaling

Abstract

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Abstract

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