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Journal of Maxillofacial and Oral Surgery

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01-03-2021 | Bone Defect | Original Article

Histopathological Examination of the Effects of Local and Systemic Bisphosphonate Usage in Bone Graft Applications on Bone Healing

Authors: Sabit Demircan, S. Cemil İşler

Published in: Journal of Maxillofacial and Oral Surgery | Issue 1/2021

Abstract

Background

The effect of bisphosphonates on the resorption process of normal bone tissue has been clearly mentioned in the literature, while their effect on the grafting material is a new research area. Limited former study is not sufficient to determine the strength, reliability and dosage of bisphosphonates. In this study, our aim is to examine the effects of local and systemic use of bisphosphonates in bone graft applications on bone healing, histopathologically.

Methods

Therefore, 32 Sprague–Dawley rats are separated into four groups. In the first group, only an empty bone defect is made on tibia and the tissue is sutured primarily without any other application. In the second group, bone defect is filled with allograft material and closed without any other application. In the third group (LA), alendronate solution is locally added to the graft material before its application to the site of bone defect. In the fourth group, alendronate is applied systemically after the site of bone defect is grafted and primarily closed. After 6 weeks, all rats are killed and the obtained samples are examined histopathologically.

Results

Local and systemic application of alendronate increases new bone formation in a statistically significant degree. In LA group, newly formed bone was observed more mature and well developed. Alendronate application does not cause an increase in inflammation, fibrosis and necrosis. There is no increased necrosis with alendronate application.

Conclusion

Local and systemic application of alendronate in bone grafting increases bone formation without any other complication. But we believe that further research should be made on dosage, usage and possible side effects.

Esposito M, Grusovin MG, Felice P, Karatzopoulos G, Worthington HV, Coulthard P (2009) The efficacy of horizontal and vertical bone augmentation procedures for dental implants—a Cochrane systematic review. Eur J Oral Implantol 2:167–184PubMed

Zouhary KJ (2010) Bone graft harvesting from distant sites: concepts and techniques. Oral Maxillofac Surg Clin N Am 22:301–316CrossRef

Cordaro L, Amade DS, Cordaro M (2011) Clinical results of alveolar ridge augmentation with mandibular block bone grafts in partially edentulous patients prior to implant placement. Clin Oral Implant Res 13:103–111CrossRef

Iwata K, Li J, Follet H, Phipps RJ, Burr DB (2006) Bisphosphonates suppress periosteal osteoblast activity independently of resorption in rat femur and tibia. Bone 39:1053–1058CrossRef

Alp YE, Taskaldiran A, Onder ME, Karahan S, Kocyigit ID, Atil F, Tekin U (2017) Effects of local low-dose alendronate injections into the distraction gap on new bone formation and distraction rate on distraction osteogenesis. J Craniofac Surg 28(8):2174–2178CrossRef

Baiomy AA, Nassan MA, Abdellatif EM, Abdel Fattah A, El-Fekey AA, Abdel Aal AB (2014) Experimental comparison of the effects of locally administered zoledronic acid and alendronate on the rate of mandibular distraction osteogenesis in dogs. Oral Surg Oral Med Oral Pathol Oral Radiol 118:35–42CrossRef

Tsuchimoto M, Azuma Y, Higuchi O, Sugimoto I, Hirata N, Kiyoki M, Yamamoto I (1994) Alendronate modulates osteogenesis of human osteoblastic cells in vitro. Jpn J Pharmacol 66:25–33CrossRef

Yaffe A, Iztkovich M, Earon Y, Alt I, Lilov R, Binderman I (1997) Local delivery of an amino bisphosphonate prevents the resorptive phase of alveolar bone following mucoperiosteal flap surgery in rats. J Periodontol 68:884–889CrossRef

Yaffe A, Binderman I, Breuer E, Pinto T, Golomb G (1999) Disposition of alendronate following local delivery in a rat jaw. J Periodontol 70:893–895CrossRef

10.

Astrand J, Aspenberg P (2004) Topical, single dose bisphosphonate treatment reduced bone resorption in a rat model for prosthetic loosening. J Orthop Res 22:244–249CrossRef

11.

Abtahi J, Agholme F, Sandberg O, Aspenberg P (2012) Bisphosphonate-induced osteonecrosis of the jaw in a rat model arises first after the bone has become exposed. No primary necrosis in unexposed bone. J Oral Pathol Med 41(6):494–499CrossRef

12.

Aspenberg P, Astrand J (2002) Bone allografts pretreated with a bisphosphonate are not resorbed. Acta Orthop Scand 73(1):20–23CrossRef

13.

Altundal H, Sayrak H, Yurtsever E, Göker K (2007) Inhibitory effect of alendronate on bone resorption of autogenous free bone grafts in rats. J Oral Maxillofac Surg 65(3):508–516CrossRef

14.

Aguirre JI, Altman MK, Vanegas SM, Franz SE, Bassit AC, Wronski TJ (2010) Effects of alendronate on bone healing after tooth extraction in rats. Oral Dis 16(7):674–685CrossRef

15.

Chavarry NGM, Perrone D, Farias MLF, Dos Santos BC, Domingos AC, Schanaider A, Feres-Filho EJ (2018) Alendronate improves bone density and type I collagen accumulation but increases the amount of pentosidine in the healing dental alveolus of ovariectomized rabbits. Bone 30(120):9–19

16.

Tanoue R, Koi K, Yamashita J (2015) Effect of Alendronate on Bone Formation during Tooth Extraction Wound Healing. J Dent Res 94(9):1251–1258CrossRef

17.

Nakamura M, Umetsu R, Abe J, Matsui T, Ueda N, Kato Y, Sasaoka S, Tahara K, Takeuchi H, Kinosada Y (2015) Analysis of the time-to-onset of osteonecrosis of jaw with bisphosphonate treatment using the data from a spontaneous reporting system of adverse drug events. J Pharm Health Care Sci 22(1):34CrossRef

18.

Srisubut S, Teerakapong A, Vattraphodes T, Taweechaisupapong S (2007) Effect of local delivery of alendronate on bone formation in bioactive glass grafting in rats. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104(4):e11–e16CrossRef

19.

Lui PP, Lee YW, Mok TY, Cheuk YC (2013) Local administration of alendronate reduced peri-tunnel bone loss and promoted graft-bone tunnel healing with minimal systemic effect on bone in contralateral knee. J Orthop Res 31(12):1897–1906CrossRef

20.

Abtahi J, Tengvall P, Aspenberg P (2012) A bisphosphonate-coating improves the fixation of metal implants in human bone. A randomized trial of dental implants. Bone 50(5):1148–1151CrossRef

21.

Kellesarian SV, Abduljabbar T, Vohra F, Malignaggi VR, Malmstrom H, Romanos GE, Javed F (2017) Role of local alendronate delivery on the osseointegration of implants: a systematic review and meta-analysis. Int J Oral Maxillofac Surg 46(7):912–921CrossRef

22.

Dundar S, Yaman F, Gecor O, Cakmak O, Kirtay M, Yildirim TT, Karaman T, Benlidayi ME (2017) Effects of local and systemic zoledronic acid application on titanium implant osseointegration: an experimental study conducted on two surface types. J Craniofac Surg 28(4):935–938CrossRef

23.

Ruggiero SL, Dodson TB, Fantasia J et al (2014) American Association of Oral and Maxillofacial Surgeons. American Association of Oral and Maxillofacial Surgeons position paper on medication-related osteonecrosis of the jaw—2014 update. J Oral Maxillofac Surg 72:1938–1956CrossRef

Title: Histopathological Examination of the Effects of Local and Systemic Bisphosphonate Usage in Bone Graft Applications on Bone Healing
Authors: Sabit Demircan
S. Cemil İşler
Publication date: 01-03-2021
Publisher: Springer India
Keywords: Bone Defect
Bisphosphonate
Published in: Journal of Maxillofacial and Oral Surgery / Issue 1/2021
Print ISSN: 0972-8279
Electronic ISSN: 0974-942X
DOI: https://doi.org/10.1007/s12663-020-01335-w

At a glance: The STEP trials

Springer Medicine

Histopathological Examination of the Effects of Local and Systemic Bisphosphonate Usage in Bone Graft Applications on Bone Healing

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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