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Canadian Journal of Anesthesia/Journal canadien d'anesthésie

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01-12-2010 | Reports of Original Investigations

Sevoflurane does not alter norepinephrine-induced intracellular Ca²⁺ changes in the diabetic rat aorta

Authors: Keisuke Fujii, MD, Koji Ogawa, MD, Yasuyuki Tokinaga, MD, Hiroshi Iranami, MD, Yoshio Hatano, MD

Published in: Canadian Journal of Anesthesia/Journal canadien d'anesthésie | Issue 12/2010

Abstract

Purpose

The effect of volatile anesthetics on the mechanism(s) of vascular contraction in diabetes mellitus (DM) has not been fully understood. The current study was designed to determine the effects of sevoflurane on the norepinephrine (NE)-induced changes in contractile state and intracellular Ca²⁺ concentrations ([Ca²⁺]_i) in the spontaneously developing type 2 DM rat.

Methods

The effects of sevoflurane on NE (10⁻⁶M)-induced vasoconstriction and increase in [Ca²⁺]_i in the aortas from Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a type 2 DM model, and from age-matched control Long-Evans Tokushima Otsuka (LETO) rats were investigated using an isometric force transducer and fluorometer with fura-2 as an indicator of [Ca²⁺]_i.

Results

Norepinephrine-induced increases in tension and [Ca²⁺]_i in OLETF rats were 54.8%, 95% confidence interval (CI) 36.9-72.6% and 58.8%, 95% CI 51.5-66.1%, respectively, and in LETO rats they were 46.4%, 95% CI 39.0-53.7% and 53.8%, 95% CI 46.9-60.7%, respectively, when expressed as the percentage relative to that induced by KCl 30 mM. In LETO rats, sevoflurane at a concentration of 3.4% inhibited the vascular contraction (9.4%, 95% CI 6.3-12.6%; P < 0.001) and the increase in [Ca²⁺]_i (33.3%, 95% CI 27.4-39.2%; P = 0.002). In OLETF rats, however, sevoflurane failed to affect either the NE-induced contraction (43.6%, 95% CI 28.3-58.9%; P = 0.68) or the elevation in [Ca²⁺]_i (60.5%, 95% CI 56.3-64.8%; P = 0.93).

Conclusion

Sevoflurane at clinically relevant concentrations inhibited the NE-induced increase in [Ca²⁺]_i in the aortic smooth muscle from normal rats but not in that from type 2 DM rats. Thus, a Ca²⁺- signalling pathway resistant to sevoflurane appears to exist in the type 2 DM rat aorta.

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Title: Sevoflurane does not alter norepinephrine-induced intracellular Ca2+ changes in the diabetic rat aorta
Authors: Keisuke Fujii, MD
Koji Ogawa, MD
Yasuyuki Tokinaga, MD
Hiroshi Iranami, MD
Yoshio Hatano, MD
Publication date: 01-12-2010
Publisher: Springer-Verlag
Published in: Canadian Journal of Anesthesia/Journal canadien d'anesthésie / Issue 12/2010
Print ISSN: 0832-610X
Electronic ISSN: 1496-8975
DOI: https://doi.org/10.1007/s12630-010-9387-0

At a glance: The STEP trials

Springer Medicine

Sevoflurane does not alter norepinephrine-induced intracellular Ca²⁺ changes in the diabetic rat aorta

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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Acknowledgement