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Canadian Journal of Anesthesia/Journal canadien d'anesthésie
Published in: Canadian Journal of Anesthesia/Journal canadien d'anesthésie 3/2009

01-03-2009 | Reports of Original Investigations

Propofol inhibits cyclo-oxygenase activity in human monocytic THP-1 cells

Authors: Takefumi Inada, MD, Kozue Kubo, MD, Tomoko Kambara, MD, Koh Shingu, MD

Published in: Canadian Journal of Anesthesia/Journal canadien d'anesthésie | Issue 3/2009

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Abstract

Purpose

Monocytes/macrophages are key players in innate and adaptive immunity. Upon stimulation, they secrete prostanoids, which are produced by cyclooxygenase from arachidonic acid. Prostanoids influence inflammation and immune responses. We investigated the effect of propofol on prostaglandin E2 and thromboxane B2 production by the human monocytic cell line THP-1.

Methods

The THP-1 cells were cultured with lipopolysaccharide (1 μg ml−1) in the presence of clinically relevant sedative/anesthetic concentrations of propofol (0–30 μM) for 18 h, and the concentration of prostaglandin E2 and thromboxane B2 in culture supernatants was measured using an enzyme immunoassay. Intracellular cyclooxygenase protein expression was measured by flow cytometry. Cyclooxygenase activity was assessed by measuring production of prostaglandin E2 and thromboxane B2 by THP-1 cells after arachidonic acid (10 μM) substrate provision.

Results

Propofol decreased the production of prostaglandin E2 (75.4 ± 6.4 pg ml−1 at 0 μM vs. 28.5 ± 11.2 pg ml−1 at 30 μM; P < 0.001) and thromboxane B2 (282.4 ± 79.2 pg ml−1 at 0 μM vs. 40.4 ± 21.7 pg ml−1 at 30 μM; P < 0.001). The inhibition was not due to the decreased cyclooxygenase protein expression because intracellular staining of this enzyme was not affected by propofol. After arachidonic acid provision, prostaglandin E2 and thromboxane B2 production from activated THP-1 cells was significantly (P < 0.001) decreased with propofol, indicating direct suppression of cyclooxygenase activity with propofol.

Conclusions

Propofol may modulate inflammation via the suppression of cyclooxygenase activity. Through the inhibition of prostanoid production, propofol may enhance immune responses.
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Metadata
Title
Propofol inhibits cyclo-oxygenase activity in human monocytic THP-1 cells
Authors
Takefumi Inada, MD
Kozue Kubo, MD
Tomoko Kambara, MD
Koh Shingu, MD
Publication date
01-03-2009
Publisher
Springer-Verlag
Published in
Canadian Journal of Anesthesia/Journal canadien d'anesthésie / Issue 3/2009
Print ISSN: 0832-610X
Electronic ISSN: 1496-8975
DOI
https://doi.org/10.1007/s12630-008-9035-0

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