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Anatomical Science International
Published in: Anatomical Science International 4/2017

01-09-2017 | Original Article

In vitro and in vivo study of microporous ceramics using MC3T3 cells, CAM assay and a pig animal model

Authors: Marek Tomco, Eva Petrovova, Maria Giretova, Viera Almasiova, Katarina Holovska, Viera Cigankova, Andrej Jenca Jr., Janka Jencova, Andrej Jenca, Martin Boldizar, Kosa Balazs, Lubomir Medvecky

Published in: Anatomical Science International | Issue 4/2017

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Abstract

Bone tissue engineering combines biomaterials with biologically active factors and cells to hold promise for reconstructing craniofacial defects. In this study the biological activity of biphasic hydroxyapatite ceramics (HA; a bone substitute that is a mixture of hydroxyapatite and β-tricalcium phosphate in fixed ratios) was characterized (1) in vitro by assessing the growth of MC3T3 mouse osteoblast lineage cells, (2) in ovo by using the chick chorioallantoic membrane (CAM) assay and (3) in an in vivo pig animal model. Biocompatibility, bioactivity, bone formation and biomaterial degradation were detected microscopically and by radiology and histology. HA ceramics alone demonstrated great biocompatibility on the CAM as well as bioactivity by increased proliferation and alkaline phosphatase secretion of mouse osteoblasts. The in vivo implantation of HA ceramics with bone marrow mesenchymal stem cells (MMSCs) showed de novo intramembranous bone healing of critical-size bone defects in the right lateral side of pig mandibular bodies after 3 and 9 weeks post-implantation. Compared with the HA ceramics without MMSCs, the progress of bone formation was slower with less-developed features. This article highlights the clinical use of microporous biphasic HA ceramics despite the unusually shaped elongated micropores with a high length/width aspect ratio (up to 20) and absence of preferable macropores (>100 µm) in bone regenerative medicine.
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Metadata
Title
In vitro and in vivo study of microporous ceramics using MC3T3 cells, CAM assay and a pig animal model
Authors
Marek Tomco
Eva Petrovova
Maria Giretova
Viera Almasiova
Katarina Holovska
Viera Cigankova
Andrej Jenca Jr.
Janka Jencova
Andrej Jenca
Martin Boldizar
Kosa Balazs
Lubomir Medvecky
Publication date
01-09-2017
Publisher
Springer Japan
Published in
Anatomical Science International / Issue 4/2017
Print ISSN: 1447-6959
Electronic ISSN: 1447-073X
DOI
https://doi.org/10.1007/s12565-016-0362-x

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