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01-09-2009

Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Brush Cells in the Adult Mouse Cerebellum

Authors: Seung-Hyuk Chung, Hassan Marzban, Masahiko Watanabe, Richard Hawkes

Published in: The Cerebellum | Issue 3/2009

Abstract

Unipolar brush cells (UBCs) are glutamatergic cerebellar interneurons of the granular layer. Previous studies have shown that there are two distinct subsets of UBCs present in the mice cerebellar cortex: calcium-binding protein calretinin (CR) positive and metabotropic glutamate receptor (mGluR)1α positive. In this study, we identify phospholipase C (PLC) β4 as an antigenic marker of a novel subset of UBCs. Double immunolabeling reveals that none of the CR+ subset expresses PLCβ4. In contrast, most members of the mGluR1α subset also express PLCβ4. In addition, 65% of the PLCβ4+ subset does not express mGluR1α. Thus, there are three distinct UBC subsets in the mouse cerebellum: CR+/PLCβ4−/mGluR1α−, PLCβ4+/mGluR1α−/CR−, and mGluR1α+/PLCβ4+/CR−. Each has a different topographical distribution, both between lobules and mediolaterally within the vermis. The development of PLCβ4 expression in UBCs is exclusively postnatal—first seen only at P12 and mature at about 3 weeks. A distinct subset of PLCβ4+ UBCs is also present in primary cerebellar cultures.

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Title: Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Brush Cells in the Adult Mouse Cerebellum
Authors: Seung-Hyuk Chung
Hassan Marzban
Masahiko Watanabe
Richard Hawkes
Publication date: 01-09-2009
Publisher: Springer-Verlag
Published in: The Cerebellum / Issue 3/2009
Print ISSN: 1473-4222
Electronic ISSN: 1473-4230
DOI: https://doi.org/10.1007/s12311-009-0092-x

At a glance: The STEP trials

Springer Medicine

Phospholipase Cβ4 Expression Identifies a Novel Subset of Unipolar Brush Cells in the Adult Mouse Cerebellum

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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