Published in:
01-04-2018 | Original Article
Predictors of Mononuclear Cell Yield in Patients Undergoing Autologous Mononuclear Stem Cell Therapy in Non-haemopoietic Degenerative Disorders
Authors:
Deepak Pahwa, Ratti Ram Sharma, Neelam Marwaha
Published in:
Indian Journal of Hematology and Blood Transfusion
|
Issue 2/2018
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Abstract
Cellular therapy outcomes are influenced by cellular composition of the product. We analyzed the cellular profiles (TNC, MNC and CD34+ cells) of patients receiving mononuclear cell therapy in terms of age, gender, BMI, pre-harvest haematological counts and clinical conditions. Cellular profiles of 262 patients were analyzed in terms of age (age < 40 year, age 41–60 year and age > 60 year), gender, BMI (BMI < 22 kg/m2, BMI 22–25 kg/m2 and BMI > 25 kg/m2), pre-harvest haematological profile and clinical conditions (chronic disorders, group A, acute vascular group B and traumatic events, group C). A steady decline was observed in TNC and MNC counts with increasing age and BMI. In clinical conditions, group C showed a highest cellular yield followed by group A and group B respectively. Amongst the three age groups, group I (age < 40 year) showed a better cellular profiles than group II (age 41–60 year) and group III (age > 60 year). Patients with Higher TLC (>7000/μl) and platelet count (>200 × 103/μl) yielded better cellular profile in the harvest. Patient age, BMI, haematological counts and clinical condition significantly affect the bone marrow cellular profile.