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Breast Cancer
Published in: Breast Cancer 6/2021

01-11-2021 | Breast Cancer | Original Article

TP53 signature diagnostic system using multiplex reverse transcription–polymerase chain reaction system enables prediction of prognosis of breast cancer patients

Authors: Shin Takahashi, Takafumi Fukui, Tadashi Nomizu, Yoichiro Kakugawa, Fumisyoshi Fujishima, Takanori Ishida, Noriaki Ohuchi, Chikashi Ishioka

Published in: Breast Cancer | Issue 6/2021

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Abstract

Background

TP53 status based on TP53 signature, a gene expression profile to determine the presence or absence of TP53 mutation, is an independent prognostic factor of breast cancer. The purpose of this study was to develop a simple diagnostic system for TP53 signature status.

Methods

We developed a multiplex reverse transcription–polymerase chain reaction system to determine TP53 status. Based on this system, prospectively collected 189 patients with stage I and II breast cancer were determined to have TP53 mutant signature or TP53 wild-type signature. The prognostic significance of the TP53 signature by the diagnostic system was analyzed.

Results

The diagnostic accuracy of TP53 status and reproducibility of this diagnosis system was confirmed. Using the diagnostic system, 89 patients were classified as TP53 mutant signature and the remaining 100 cases were classified as TP53 wild-type signature. Recurrence-free survival (RFS) among patients with TP53 mutant signature was significantly shorter than that among those with TP53 wild-type signature. On univariate and multivariate analyses, the TP53 signature status was an independent predictor of RFS. RFS among patients with TP53 mutant signature was significantly shorter than that among those with TP53 wild-type signature in a cohort of estrogen receptor-positive breast cancer. Although a difference was not significant, no recurrent cases was observed in TP53 wild-type signature group in triple negative breast cancer.

Conclusion

This simple and precise diagnostic system to determine TP53 signature status may help in prognostic assessment, therapeutic decision-making, and treatment optimization in patients with breast cancer.
Appendix
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Metadata
Title
TP53 signature diagnostic system using multiplex reverse transcription–polymerase chain reaction system enables prediction of prognosis of breast cancer patients
Authors
Shin Takahashi
Takafumi Fukui
Tadashi Nomizu
Yoichiro Kakugawa
Fumisyoshi Fujishima
Takanori Ishida
Noriaki Ohuchi
Chikashi Ishioka
Publication date
01-11-2021
Publisher
Springer Singapore
Published in
Breast Cancer / Issue 6/2021
Print ISSN: 1340-6868
Electronic ISSN: 1880-4233
DOI
https://doi.org/10.1007/s12282-021-01250-z

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