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01-04-2014 | Original Article

Effect of Leptin and Apelin Preconditioning on Hepatic Ischemia Reperfusion Injury in Rats

Authors: Tamer Sagiroglu, Mustafa Burak Aksoy, Gonul Sagiroglu, Hilmi Tozkir, Serhat Oguz, Tulin Yalta, Mehmet A. Yagci, Atakan Sezer

Published in: Indian Journal of Surgery | Issue 2/2014

Abstract

Leptin and apelin are important adipocytokines involved in a variety of endocrine and paracrine functions. The aim of this study was to evaluate the effect of exogenous leptin and apelin preconditioning on hepatic ischemia reperfusion (I/R) injury in rats. Forty mice were assigned to four groups (n = 10): sham-operated control (sham), I/R injury, I/R + leptin (I/R + L), and I/R + apelin (I/R + A). Leptin 100 μg/kg/day and apelin 2 μg/kg/day were delivered intraperitoneally starting 3 days prior to surgical procedure in I/R + L and I/R + A groups, respectively. All I/R groups underwent 45 min of warm ischemia, followed by 30 min of reperfusion. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver malondialdehyde (MDA) and glutathione (GSH), and liver histopathology were compared between groups. MDA was elevated in I/R, but stayed similar in I/R + L and I/R + A compared to sham. I/R + A had significantly lower MDA compared to I/R. GSH levels did not differ significantly between the groups. ALT and AST were elevated in all I/R groups, but significant reduction was observed in I/R + L and I/R + A compared to I/R. Liver histopathology was mostly mild in I/R + L and I/R + A, in contrast to severe injury observed in the I/R group. Leptin and apelin preconditioning significantly reduced hepatic I/R injury in rats.

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Title: Effect of Leptin and Apelin Preconditioning on Hepatic Ischemia Reperfusion Injury in Rats
Authors: Tamer Sagiroglu
Mustafa Burak Aksoy
Gonul Sagiroglu
Hilmi Tozkir
Serhat Oguz
Tulin Yalta
Mehmet A. Yagci
Atakan Sezer
Publication date: 01-04-2014
Publisher: Springer India
Published in: Indian Journal of Surgery / Issue 2/2014
Print ISSN: 0972-2068
Electronic ISSN: 0973-9793
DOI: https://doi.org/10.1007/s12262-012-0676-4

At a glance: The STEP trials

Springer Medicine

Effect of Leptin and Apelin Preconditioning on Hepatic Ischemia Reperfusion Injury in Rats

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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