Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Pathology & Oncology Research
Published in: Pathology & Oncology Research 1/2019

01-01-2019 | Original Article

A Potential Role for Green Tea as a Radiation Sensitizer for Prostate Cancer

Authors: Andrew C. Schroeder, Huaping Xiao, Ziwen Zhu, Qing Li, Qian Bai, Mark R. Wakefield, Jeffrey D. Mann, Yujiang Fang

Published in: Pathology & Oncology Research | Issue 1/2019

Login to get access

Abstract

Prostate cancer (PCa) is the most common non-cutaneous cancer in the United States. There is currently a lack of safe and effective radiosensitizers that can enhance the effectiveness of radiation treatment (RT) for Pca. Clonogenic assay, PCNA staining, Quick Cell Proliferation assay, TUNEL staining and caspase-3 activity assay were used to assess proliferation and apoptosis in DU145 Pca cells. RT-PCR/IHC were used to investigate the mechanisms. We found that the percentage of colonies, PCNA staining intensity, and the optical density value of DU145 cells were decreased (RT/GT vs. RT). TUNEL + cells and the relative caspase-3 activity were increased (RT/GT vs. RT). Compared to RT, the anti-proliferative effect of RT/GT correlated with increased expression of the anti-proliferative molecule p16. Compared to RT, the pro-apoptotic effect of RT/GT correlated with decreased expression of the anti-apoptotic molecule Bcl-2. GT enhances RT sensitivity of DU145 by inhibiting proliferation and promoting apoptosis.
Literature
1.
2.
Bernard W, Christopher P (2014) World cancer report 2014. International Agency for Research on Cancer. World Health Organization, Lyon, France
3.
Zhang S, Shan L, Li Q, Wang X, Li S, Zhang Y, Fu J, Liu X, Li H, Zhang W (2014) Systematic analysis of the multiple bioactivities of green tea through a network pharmacology approach. Evid Based Complement Alternat Med 2014:512081. https://​doi.​org/​10.​1155/​2014/​512081
4.
Fang Y, DeMarco VG, Nicholl MB (2012) Resveratrol enhances radiation sensitivity in prostate cancer by inhibiting cell proliferation and promoting cell senescence and apoptosis. Cancer Sci 103(6):1090–1098CrossRefPubMed
5.
6.
Liang X, Gao J, Sun X, Zhu L, Jia Y, Gu Y, Han C, Zhang X, Hou S (2013) [Tea polyphenols inhibits the proliferation of prostate cancer DU145 cells]. Zhonghua nan ke xue=. Natl J Androl 19(6):495–500
7.
Kwak TW, Park SB, Kim H-J, Jeong Y-I, Kang DH (2017) Anticancer activities of epigallocatechin-3-gallate against cholangiocarcinoma cells. OncoTargets Ther 10:137CrossRef
8.
Naponelli V, Ramazzina I, Lenzi C, Bettuzzi S, Rizzi F (2017) Green tea Catechins for prostate cancer prevention: present achievements and future challenges. Antioxidants 6(2):26CrossRefPubMedCentral
9.
Oya Y, Mondal A, Rawangkan A, Umsumarng S, Iida K, Watanabe T, Kanno M, Suzuki K, Li Z, Kagechika H, Shudo K, Fujiki H, Suganuma M (2017) Down-regulation of histone deacetylase 4, −5 and −6 as a mechanism of synergistic enhancement of apoptosis in human lung cancer cells treated with the combination of a synthetic retinoid, Am80 and green tea catechin. J Nutr Biochem 42:7–16. https://​doi.​org/​10.​1016/​j.​jnutbio.​2016.​12.​015 CrossRefPubMed
10.
Tsai Y-J, Chen B-H (2016) Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3. Int J Nanomedicine 11:1907CrossRefPubMedPubMedCentral
11.
Henning SM, Aronson W, Niu Y, Conde F, Lee NH, Seeram NP, Lee R-P, Lu J, Harris DM, Moro A (2006) Tea polyphenols and theaflavins are present in prostate tissue of humans and mice after green and black tea consumption. J Nutr 136(7):1839–1843CrossRefPubMed
12.
Fang Y, Bradley MJ, Cook KM, Herrick EJ, Nicholl MB (2013) A potential role for resveratrol as a radiation sensitizer for melanoma treatment. J Surg Res 183(2):645–653CrossRefPubMed
13.
Fang Y, Braley-Mullen H (2008) Cultured murine thyroid epithelial cells expressing transgenic Fas-associated death domain-like interleukin-1β converting enzyme inhibitory protein are protected from Fas-mediated apoptosis. Endocrinology 149(7):3321–3329CrossRefPubMedPubMedCentral
14.
Fang Y, Sharp GC, Braley-Mullen H (2008) Interleukin-10 promotes resolution of granulomatous experimental autoimmune thyroiditis. Am J Pathol 172(6):1591–1602CrossRefPubMedPubMedCentral
15.
Fang Y, Wei Y, DeMarco V, Chen K, Sharp GC, Braley-Mullen H (2007) Murine FLIP transgene expressed on thyroid epithelial cells promotes resolution of granulomatous experimental autoimmune thyroiditis in DBA/1 mice. Am J Pathol 170(3):875–887CrossRefPubMedPubMedCentral
16.
Fang Y, Moore BJ, Bai Q, Cook KM, Herrick EJ, Nicholl MB (2013) Hydrogen peroxide enhances radiation-induced apoptosis and inhibition of melanoma cell proliferation. Anticancer Res 33(5):1799–1807PubMed
17.
Fang Y, Herrick EJ, Nicholl MB (2012) A possible role for perforin and Granzyme B in resveratrol-enhanced Radiosensitivity of prostate cancer. J Androl 33(4):752–760CrossRefPubMed
18.
Fang Y, Sharp GC, Yagita H, Braley-Mullen H (2008) A critical role for TRAIL in resolution of granulomatous experimental autoimmune thyroiditis. J Pathol 216(4):505–513CrossRefPubMedPubMedCentral
19.
20.
Johnson D, Walker C (1999) Cyclins and cell cycle checkpoints. Annu Rev Pharmacol Toxicol 39(1):295–312CrossRef
21.
Nobori T (1994) Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Trends Genet 10(7):228CrossRef
22.
Helgadottir H, Höiom V, Jönsson G, Tuominen R, Ingvar C, Borg Å, Olsson H, Hansson J (2014) High risk of tobacco-related cancers in CDKN2A mutation-positive melanoma families. J Med Genet 51(8):545–552CrossRefPubMedPubMedCentral
23.
Caldas C, Hahn SA, da Costa LT, Redston MS, Schutte M, Seymour AB, Weinstein CL, Hruban RH, Yeo CJ, Kern SE (1994) Frequent somatic mutations and homozygous deletions of the p16 (MTS1) gene in pancreatic adenocarcinoma. Nat Genet 8(1):27–32CrossRefPubMed
24.
Griffith TS, Brunner T, Fletcher SM, Green DR, Ferguson TA (1995) Fas ligand-induced apoptosis as a mechanism of immune privilege. Science 270(5239):1189–1192CrossRefPubMed
25.
Thompson CB (1995) Apoptosis in the pathogenesis and treatment of disease. Science 267(5203):1456CrossRef
26.
27.
Zhang H, Xu Q, Krajewski S, Krajewska M, Xie Z, Fuess S, Kitada S, Pawłowski K, Godzik A, Reed JC (2000) BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins. Proc Natl Acad Sci 97(6):2597–2602CrossRefPubMed
28.
29.
Khanna K, Wie T, Song Q, Burrows S, Moss D, Krajewski S, Reed J, Lavin M (1996) Expression of p53, bcl-2, bax, bcl-x2 and c-myc in radiation-induced apoptosis in Burkitt's lymphoma cells. Cell Death Differ 3(3):315–322PubMed
30.
Krajewska M, Krajewski S, Epstein JI, Shabaik A, Sauvageot J, Song K, Kitada S, Reed JC (1996) Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. Am J Pathol 148(5):1567PubMedPubMedCentral
31.
Tron V, Krajewski S, Klein-Parker H, Li G, Ho V, Reed J (1995) Immunohistochemical analysis of Bcl-2 protein regulation in cutaneous melanoma. Am J Pathol 146(3):643PubMedPubMedCentral
32.
Tsujimoto Y, Cossman J, Jaffe E, Croce CM (1985) Involvement of the bcl-2 gene in human follicular lymphoma. Science 228(4706):1440–1443CrossRefPubMed
33.
Yip KW, Shi W, Pintilie M, Martin JD, Mocanu JD, Wong D, MacMillan C, Gullane P, O'Sullivan B, Bastianutto C (2006) Prognostic significance of the Epstein-Barr virus, p53, Bcl-2, and survivin in nasopharyngeal cancer. Clin Cancer Res 12(19):5726–5732CrossRefPubMed
34.
Zhu Z, Davidson KT, Brittingham A, Wakefield MR, Bai Q, Xiao H, Fang Y (2016) Trichomonas vaginalis: a possible foe to prostate cancer. Med Oncol 33(10):115CrossRefPubMed
Metadata
Title
A Potential Role for Green Tea as a Radiation Sensitizer for Prostate Cancer
Authors
Andrew C. Schroeder
Huaping Xiao
Ziwen Zhu
Qing Li
Qian Bai
Mark R. Wakefield
Jeffrey D. Mann
Yujiang Fang
Publication date
01-01-2019
Publisher
Springer Netherlands
Published in
Pathology & Oncology Research / Issue 1/2019
Print ISSN: 1219-4956
Electronic ISSN: 1532-2807
DOI
https://doi.org/10.1007/s12253-017-0358-4

Other articles of this Issue 1/2019

Pathology & Oncology Research 1/2019 Go to the issue

Original Article

MicroRNA Expression in Laser Micro-dissected Breast Cancer Tissue Samples – a Pilot Study

Original Article

The Impact of Peritumoral Retraction Clefting & Intratumoral Eosinophils on Overall Survival in Oral Squamous Carcinoma Patients

Original Article

Mutational Diversity of Lung Cancer and Associated Lymph Nodes. An Exploratory Prospective Study of 4 Resected cIIIA-N2

Original Article

Overexpression of TIMP-1 and Sensitivity to Topoisomerase Inhibitors in Glioblastoma Cell Lines

Original Article

RETRACTED ARTICLE: Effects of microRNA-708 on Epithelial-Mesenchymal Transition, Cell Proliferation and Apoptosis in Melanoma Cells by Targeting LEF1 through the Wnt Signaling Pathway

Original Article

The Significance of Long Non-coding RNA HULC in Predicting Prognosis and Metastasis of Cancers: a Meta-Analysis
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits