Top

Published in:

01-04-2015 | Research

Lantana camara Induces Apoptosis by Bcl-2 Family and Caspases Activation

Authors: Eun Byeol Han, Bo Yoon Chang, Young Suk Jung, Sung Yeon Kim

Published in: Pathology & Oncology Research | Issue 2/2015

Abstract

Breast cancer is one of the most common cancers worldwide, and the second most fatal cancer in women after lung cancer. Because there are instances of cancer resistance to existing therapies, studies focused on the identification of novel therapeutic drugs are very important. In this study, we identified a natural anticancer agent from Lantana camara, a flowering plant species of the genus Verbena. The extract obtained from the L. camara exhibited cell death properties in the human breast cancer cell line, MCF-7. We found that the apoptosis induced by treatment with the L. camara extract was regulated by the Bcl-2 family. Bid and Bax was increased and Bcl-2 was decreased by L. camara extract. L. camara extract modulated cleavage of caspase-8, and caspase-9, as well as poly (ADP-ribose) polymerase (PARP). Our results support the potential use of the L. camara extract as an anti-breast cancer drug.

Adams JM, Cory S (2007) The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene 26(9):1324–1337. doi:10.1038/sj.onc.1210220 CrossRefPubMedCentralPubMed

Almubarak H, Jones A, Chaisuparat R, Zhang M, Meiller TF, Scheper MA (2011) Zoledronic acid directly suppresses cell proliferation and induces apoptosis in highly tumorigenic prostate and breast cancers. J of carcinog 10:2. doi:10.4103/1477-3163.75723 CrossRef

Bentzon N, During M, Rasmussen BB, Mouridsen H, Kroman N (2008) Prognostic effect of estrogen receptor status across age in primary breast cancer. Int j of cancer J int du cancer 122(5):1089–1094. doi:10.1002/ijc.22892 CrossRef

Chen YH, Yeh CW, Lo HC, Su SL, Hseu YC, Hsu LS (2012) Generation of reactive oxygen species mediates butein-induced apoptosis in neuroblastoma cells. Oncol Rep 27(4):1233–1237. doi:10.3892/or.2012.1632 PubMedCentralPubMed

Cory S, Huang DC, Adams JM (2003) The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 22(53):8590–8607. doi:10.1038/sj.onc.1207102 CrossRefPubMed

Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13(8):795–808. doi:10.1002/cyto.990130802 CrossRefPubMed

Deena MJ, Thoppil JE (2000) Antimicrobial activity of the essential oil of Lantana camara. Fitoterapia 71(4):453–455CrossRefPubMed

Dunnwald LK, Rossing MA, Li CI (2007) Hormone receptor status, tumor characteristics, and prognosis: a prospective cohort of breast cancer patients. Breast cancer res : BCR 9(1):R6. doi:10.1186/bcr1639 CrossRefPubMedCentralPubMed

Fan TJ, Han LH, Cong RS, Liang J (2005) Caspase family proteases and apoptosis. Acta Biochim Biophys Sin 37(11):719–727CrossRefPubMed

10.

Fulda S (2010) Modulation of apoptosis by natural products for cancer therapy. Planta Med 76(11):1075–1079. doi:10.1055/s-0030-1249961 CrossRefPubMed

11.

Ghisalberti EL (2000) Lantana camara L. (Verbenaceae). Fitoterapia 71(5):467–486CrossRefPubMed

12.

Ghobrial IM, Witzig TE, Adjei AA (2005) Targeting apoptosis pathways in cancer therapy. CA: a cancer j for clin 55(3):178–194

13.

Heeres JT, Hergenrother PJ (2007) Poly(ADP-ribose) makes a date with death. Curr Opin Chem Biol 11(6):644–653. doi:10.1016/j.cbpa.2007.08.038 CrossRefPubMed

14.

Jonville MC, Kodja H, Humeau L, Fournel J, De Mol P, Cao M, Angenot L, Frederich M (2008) Screening of medicinal plants from Reunion Island for antimalarial and cytotoxic activity. J Ethnopharmacol 120(3):382–386. doi:10.1016/j.jep.2008.09.005 CrossRefPubMed

15.

Kasibhatla S, Tseng B (2003) Why target apoptosis in cancer treatment? Mol Cancer Ther 2(6):573–580PubMed

16.

Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirier GG (1993) Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res 53(17):3976–3985PubMed

17.

Kumar VP, Chauhan NS, Padh H, Rajani M (2006) Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol 107(2):182–188. doi:10.1016/j.jep.2006.03.013 CrossRefPubMed

18.

Nayak BS, Raju SS, Eversley M, Ramsubhag A (2009) Evaluation of wound healing activity of Lantana camara L. - a preclinical study. Phytother res : PTR 23(2):241–245. doi:10.1002/ptr.2599 CrossRefPubMed

19.

Nguewa PA, Fuertes MA, Alonso C, Perez JM (2003) Pharmacological modulation of Poly(ADP-ribose) polymerase-mediated cell death: exploitation in cancer chemotherapy. Mol Pharmacol 64(5):1007–1014. doi:10.1124/mol.64.5.1007 CrossRefPubMed

20.

Nita ME, Nagawa H, Tominaga O, Tsuno N, Fujii S, Sasaki S, Fu CG, Takenoue T, Tsuruo T, Muto T (1998) 5-Fluorouracil induces apoptosis in human colon cancer cell lines with modulation of Bcl-2 family proteins. Br J Cancer 78(8):986–992CrossRefPubMedCentralPubMed

21.

Niu M, Sun Y, Liu X, Tang L, Qiu R (2013) Tautomycetin induces apoptosis by inactivating Akt through a PP1-independent signaling pathway in human breast cancer cells. J Pharmacol Sci 121(1):17–24CrossRefPubMed

22.

Oliver FJ, de la Rubia G, Rolli V, Ruiz-Ruiz MC, de Murcia G, Murcia JM (1998) Importance of poly(ADP-ribose) polymerase and its cleavage in apoptosis. Lesson from an uncleavable mutant. The J of biol chem 273(50):33533–33539CrossRef

23.

Partridge AH, Burstein HJ, Winer EP (2001) Side effects of chemotherapy and combined chemohormonal therapy in women with early-stage breast cancer. J Natl Cancer Inst Monogr 30:135–142CrossRefPubMed

24.

Shapiro CL, Recht A (2001) Side effects of adjuvant treatment of breast cancer. N Engl J Med 344(26):1997–2008. doi:10.1056/NEJM200106283442607 CrossRefPubMed

25.

Sharma M, Sharma PD, Bansal MP (2008) Lantadenes and their esters as potential antitumor agents. J Nat Prod 71(7):1222–1227. doi:10.1021/np800167x CrossRefPubMed

26.

Sharma M, Sharma PD, Bansal MP, Singh J (2007) Synthesis, cytotoxicity, and antitumor activity of lantadene-A congeners. Chem & biodivers 4(5):932–939. doi:10.1002/cbdv.200790082 CrossRef

27.

Siegel R, Naishadham D, Jemal A (2012) Cancer statistics, 2012. CA: a cancer j for clin 62(1):10–29. doi:10.3322/caac.20138

28.

Sun SN, Jia WD, Chen H, Ma JL, Ge YS, Yu JH, Li JS (2013) Docosahexaenoic acid (DHA) induces apoptosis in human hepatocellular carcinoma cells. Int j of clin and exp pathol 6(2):281–289

29.

Tempfer CB, Froese G, Heinze G, Bentz EK, Hefler LA, Huber JC (2009) Side effects of phytoestrogens: a meta-analysis of randomized trials. The Am j of med 122(10):939–946. doi:10.1016/j.amjmed.2009.04.018 CrossRef

30.

Tsujimoto Y (1998) Role of Bcl-2 family proteins in apoptosis: apoptosomes or mitochondria? Genes to cells : devoted to mol & cell mech 3(11):697–707CrossRef

31.

Weis J, Poppelreuter M, Bartsch HH (2009) Cognitive deficits as long-term side-effects of adjuvant therapy in breast cancer patients: ‘subjective’ complaints and ‘objective’ neuropsychological test results. Psycho-Oncology 18(7):775–782. doi:10.1002/pon.1472 CrossRefPubMed

32.

Yang L, Wu D, Luo K, Wu S, Wu P (2009) Andrographolide enhances 5-fluorouracil-induced apoptosis via caspase-8-dependent mitochondrial pathway involving p53 participation in hepatocellular carcinoma (SMMC-7721) cells. Cancer Lett 276(2):180–188. doi:10.1016/j.canlet.2008.11.015 CrossRefPubMed

Title: Lantana camara Induces Apoptosis by Bcl-2 Family and Caspases Activation
Authors: Eun Byeol Han
Bo Yoon Chang
Young Suk Jung
Sung Yeon Kim
Publication date: 01-04-2015
Publisher: Springer Netherlands
Published in: Pathology & Oncology Research / Issue 2/2015
Print ISSN: 1219-4956
Electronic ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-014-9824-4

Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras

Prof. Fabrice Barlesi

Developed by: Springer Medicine

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2015

The Evaluation of WBP2NL-Related Genes Expression in Breast Cancer

Correlation Between E-cadherin Immunoexpression and Efficacy of First Line Platinum-Based Chemotherapy in Advanced High Grade Serous Ovarian Cancer

High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma

Genetic Polymorphism of CAT C-262 T and Susceptibility to Breast Cancer, a Case–Control Study and Meta-Analysis of the Literatures

Immunohistochemical Study of Glypican-3 and HepPar-1 in Differentiating Hepatocellular Carcinoma from Metastatic Carcinomas in FNA of the Liver

Accuracy and Reproducibility of HER2 Status in Breast Cancer Using Immunohistochemistry: A Quality Control Study in Tuscany Evaluating the Impact of Updated 2013 ASCO/CAP Recommendations

Keynote webinar | Spotlight on antibody–drug conjugates in cancer