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Environmental Health and Preventive Medicine
Published in: Environmental Health and Preventive Medicine 3/2012

01-05-2012 | Short Communication

Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cells

Authors: Noriyuki Nishimura, Tri Budi Hartomo, Thi Van Huyen Pham, Myeong Jin Lee, Tomoto Yamamoto, Satoru Morikawa, Daiichiro Hasegawa, Hiroki Takeda, Keiichiro Kawasaki, Yoshiyuki Kosaka, Nobuyuki Yamamoto, Ikuko Kubokawa, Takeshi Mori, Tomoko Yanai, Akira Hayakawa, Yasuhiro Takeshima, Kazumoto Iijima, Masafumi Matsuo, Hisahide Nishio

Published in: Environmental Health and Preventive Medicine | Issue 3/2012

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Abstract

Objectives

A growing number of epidemiological studies have demonstrated that the consumption of green tea inhibits the growth of a variety of cancers. Epigallocatechin gallate (EGCG), the most abundant catechin in green tea, has been shown to have an anti-cancer effect against many cancers. Most cancers are believed to be initiated from and maintained by a small population of tumor-initiating cells (TICs) that are responsible for chemotherapeutic resistance and tumor relapse. In neuroblastoma, an aggressive pediatric tumor that often relapses and has a poor prognosis, TICs were recently identified as spheres grown in a serum-free non-adherent culture used for neural crest stem cell growth. Although EGCG has been reported to induce growth arrest and apoptosis in neuroblastoma cells, its effect on neuroblastoma TICs remains to be defined.

Methods

Gene expression was analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). The effects of EGCG on cell proliferation, apoptosis, and sphere formation were determined by cell counting, propidium iodide staining, and sphere (>100 μm in diameter) counting, respectively.

Results

Neuroblastoma BE(2)-C cells showed increased expression of stem cell markers (nanog homeobox [NANOG] and octamer-binding transcription factor 4 [OCT4]), as well as decreased expression of neuronal differentiation markers (Cu2+-transporting ATPase alpha polypeptide [ATP7A] and dickkopf homolog 2 [DKK2]) in spheres grown in serum-free non-adherent culture, compared to parental cells grown in conventional culture. Although EGCG induced growth arrest and apoptosis in the parental cells in a dose-dependent manner, it was not effective against spheres. However, EGCG potently inhibited sphere formation in the BE(2)-C cells.

Conclusions

The present results suggest that EGCG may inhibit the development of TICs in BE(2)-C cells.
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Metadata
Title
Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cells
Authors
Noriyuki Nishimura
Tri Budi Hartomo
Thi Van Huyen Pham
Myeong Jin Lee
Tomoto Yamamoto
Satoru Morikawa
Daiichiro Hasegawa
Hiroki Takeda
Keiichiro Kawasaki
Yoshiyuki Kosaka
Nobuyuki Yamamoto
Ikuko Kubokawa
Takeshi Mori
Tomoko Yanai
Akira Hayakawa
Yasuhiro Takeshima
Kazumoto Iijima
Masafumi Matsuo
Hisahide Nishio
Publication date
01-05-2012
Publisher
Springer Japan
Published in
Environmental Health and Preventive Medicine / Issue 3/2012
Print ISSN: 1342-078X
Electronic ISSN: 1347-4715
DOI
https://doi.org/10.1007/s12199-011-0239-5

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