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Head and Neck Pathology
Published in: Head and Neck Pathology 3/2020

01-09-2020 | Original Paper

Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

Authors: Gopikrishnan Vijayakumar, Anjali Narwal, Mala Kamboj, Rajeev Sen

Published in: Head and Neck Pathology | Issue 3/2020

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Abstract

The cancer stem cells deliver uncontrolled proliferative capacity within the tumor imparting to increasing size while epithelial mesenchymal transition adds to the invasive potential. Studies using specific markers elucidating the role of these phenomena may bring advancement in the targeted therapy of tumor. SOX2 and OCT4 are two among few stem cell markers indicative of proliferative potential and WNT5A is an epithelial mesenchymal transition marker indicative of invasive potential. We aimed to determine the association between expression of SOX2, OCT4 and WNT5A in oral epithelial dysplasia, oral squamous cell carcinoma and normal oral mucosa. 20 cases of oral squamous cell carcinoma, 20 cases of oral epithelial dysplasia (leukoplakia with dysplasia) and 25 normal oral mucosa tissues specimens were immunohistochemically stained to assess SOX2, OCT4 and WNT5A expression. SOX2 expression was higher in oral squamous cell carcinoma than in oral epithelial dysplasia and very low in normal oral mucosa. OCT4 was very low in oral squamous cell carcinoma and oral epithelial dysplasia when compared to SOX2, while negative in normal tissues. Co-expression of SOX2 and OCT4 showed statistically non-significant difference for tumor proliferation. WNT5A expression was found to be increasing from normal oral mucosa to oral epithelial dysplasia and oral squamous cell carcinoma. In conformity with present study, SOX2 itself can act as a potential marker for proliferation in tumor cells while OCT4 has non-significant role in regulation of tumor behavior in oral squamous cell carcinoma as well as in oral epithelial dysplasia. WNT5A can be a putative marker in studying invasive potential of oral squamous cell carcinoma.
Literature
1.
Reya T, Morrison SJ, Clarke MF, Weissman IL. Stem cells, cancer, and cancer stem cells. Nature. 2001;414(6859):105.CrossRefPubMed
2.
3.
Soltysova A, Altanerova V, Altaner C. Cancer stem cells. Neoplasma. 2005;52(6):435.PubMed
4.
5.
6.
Monroe MM, Anderson EC, Clayburgh DR, Wong MH. Cancer stem cells in head and neck squamous cell carcinoma. J Oncol 2011;2011:762780.CrossRefPubMed
7.
Kaufhold S, Garbán H, Bonavida B. Yin Yang 1 is associated with cancer stem cell transcription factors (SOX2, OCT4, BMI1) and clinical implication. J Exp Clin Cancer Res. 2016;35(1):84.CrossRefPubMedPubMedCentral
8.
Alison MR, Lim SM, Nicholson LJ. Cancer stem cells: problems for therapy? J Pathol. 2011;223(2):148–62.CrossRef
9.
Costea DE, Tsinkalovsky O, Vintermyr OK, Johannessen AC, Mackenzie IC. Cancer stem cells–new and potentially important targets for the therapy of oral squamous cell carcinoma. Oral Dis. 2006;12(5):443–54.CrossRefPubMed
10.
11.
Fu TY, Hsieh IC, Cheng JT, Tsai MH, Hou YY, Lee JH, Liou HH, Huang SF, Chen HC, Yen LM, Tseng HH. Association of OCT 4, SOX 2, and NANOG expression with oral squamous cell carcinoma progression. J Oral Pathol Med. 2016;45(2):89–95.CrossRefPubMed
12.
Luo W, Li S, Peng B, Ye Y, Deng X, Yao K. Embryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma. PLoS ONE. 2013;8(2):e56324.CrossRefPubMedPubMedCentral
13.
Qiao B, He B, Cai J, Yang W. The expression profile of Oct4 and Sox2 in the carcinogenesis of oral mucosa. Int J Clin Exp Pathol. 2014;7(1):28.PubMed
14.
Lazarevic M, Milosevic M, Trisic D, Toljic B, Simonovic J, Nikolic N, Mikovic N, Jelovac D, Petrovic M, Vukadinovic M, Milasin J. Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. J BU ON. 2018;23(6):1686–92.
15.
16.
Acloque H, Adams MS, Fishwick K, Bronner-Fraser M, Nieto MA. Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease. J Clin Investig. 2009;119(6):1438–49.CrossRefPubMedPubMedCentral
17.
Katoh M. WNT/PCP signaling pathway and human cancer. Oncol Rep. 2005;14(6):1583–8.PubMed
18.
Prgomet Z, Axelsson L, Lindberg P, Andersson T. Migration and invasion of oral squamous carcinoma cells is promoted by WNT 5A, a regulator of cancer progression. J Oral Pathol Med. 2015;44(10):776–84.CrossRefPubMed
19.
Bo H, Zhang S, Gao L, Chen Y, Zhang J, Chang X, Zhu M. Upregulation of Wnt5a promotes epithelial-to-mesenchymal transition and metastasis of pancreatic cancer cells. BMC Cancer. 2013;13(1):496.CrossRefPubMedPubMedCentral
20.
Medrek C, Landberg G, Andersson T, Leandersson K. Wnt-5a-CKIα signaling promotes β-catenin/E-cadherin complex formation and intercellular adhesion in human breast epithelial cells. J Biol Chem. 2009;284(16):10968–79.CrossRefPubMedPubMedCentral
21.
Dissanayake SK, Wade M, Johnson CE, O'Connell MP, Leotlela PD, French AD, Shah KV, Hewitt KJ, Rosenthal DT, Indig FE, Jiang Y. The Wnt5A/protein kinase C pathway mediates motility in melanoma cells via the inhibition of metastasis suppressors and initiation of an epithelial to mesenchymal transition. J Biol Chem. 2007;282(23):17259–71.CrossRefPubMed
22.
Prgomet Z, Andersson T, Lindberg P. Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance. Eur J Oral Sci. 2017;125(4):237–46.CrossRefPubMedPubMedCentral
23.
Lee SH, Oh SY, Do SI, Lee HJ, Kang HJ, Rho YS, Bae WJ, Lim YC. SOX2 regulates self-renewal and tumorigenicity of stem-like cells of head and neck squamous cell carcinoma. Br J Cancer. 2014;111(11):2122.CrossRefPubMedPubMedCentral
24.
Du L, Yang Y, Xiao X, Wang C, Zhang X, Wang L, Zhang X, Li W, Zheng G, Wang S, Dong Z. Sox2 nuclear expression is closely associated with poor prognosis in patients with histologically node-negative oral tongue squamous cell carcinoma. Oral Oncol. 2011;47(8):709–13.CrossRefPubMed
25.
Huang CF, Xu XR, Wu TF, Sun ZJ, Zhang WF. Correlation of ALDH 1, CD 44, OCT 4 and SOX 2 in tongue squamous cell carcinoma and their association with disease progression and prognosis. J Oral Pathol Med. 2014;43(7):492–8.CrossRefPubMed
26.
Ge N, Lin HX, Xiao XS, Guo L, Xu HM, Wang X, Jin T, Cai XY, Liang Y, Hu WH, Kang T. Prognostic significance of Oct4 and Sox2 expression in hypopharyngeal squamous cell carcinoma. J Transl Med. 2010;8(1):94.CrossRefPubMedPubMedCentral
27.
Wang QI, He W, Lu C, Wang Z, Wang J, Giercksky KE, Nesland JM, Suo Z. Oct3/4 and Sox2 are significantly associated with an unfavorable clinical outcome in human esophageal squamous cell carcinoma. Anticancer Res. 2009;29(4):1233–41.PubMed
28.
Yu HH, Featherston T, Tan ST, Chibnall AM, Brasch HD, Davis PF, Itinteang T. Characterization of cancer stem cells in moderately differentiated buccal mucosal squamous cell carcinoma. Front Surg. 2016;3:46.CrossRefPubMedPubMedCentral
29.
Cai J, He B, Li X, Sun M, Lam AK, Qiao B, Qiu W. Regulation of tumorigenesis in oral epithelial cells by defined reprogramming factors Oct4 and Sox2. Oncol Rep. 2016;36(2):651–8.CrossRefPubMedPubMedCentral
Metadata
Title
Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study
Authors
Gopikrishnan Vijayakumar
Anjali Narwal
Mala Kamboj
Rajeev Sen
Publication date
01-09-2020
Publisher
Springer US
Published in
Head and Neck Pathology / Issue 3/2020
Electronic ISSN: 1936-0568
DOI
https://doi.org/10.1007/s12105-019-01114-1

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