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Clinical and Translational Oncology

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01-06-2015 | Research Article

Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion

Authors: X. Song, L. Hua, Y. Xu, Z. Fang, Y. Wang, J. Gao, Q. Shi, X. Zhou, R. Yu

Published in: Clinical and Translational Oncology | Issue 6/2015

Abstract

Purpose

The present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.

Methods

Wound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.

Results

We found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.

Conclusions

These findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.

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Title: Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion
Authors: X. Song
L. Hua
Y. Xu
Z. Fang
Y. Wang
J. Gao
Q. Shi
X. Zhou
R. Yu
Publication date: 01-06-2015
Publisher: Springer Milan
Published in: Clinical and Translational Oncology / Issue 6/2015
Print ISSN: 1699-048X
Electronic ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-014-1263-x

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Abstract

Purpose

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Conclusions

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