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Clinical and Translational Oncology

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01-02-2014 | Research Article

Proteomic analysis of pleural effusion from lung adenocarcinoma patients by shotgun strategy

Authors: Shu-hong Sheng, Hui-li Zhu

Published in: Clinical and Translational Oncology | Issue 2/2014

Abstract

Purpose

To construct a protein catalogue of malignant pleural effusion from lung adenocarcinoma patients and to screen the potential candidates of biomarkers for diagnostic value in human lung adenocarcinoma.

Method

Five malignant pleural effusion samples of lung adenocarcinoma patients were collected from January 2009 to September. A composite sample was analyzed using shotgun strategy. Pleural effusion samples were separated by means of SDS-PAGE. Proteomic analysis was performed by 1D-LC–MS/MS, and then the proteins were identified using SEQUEST software and protein database search.

Results

Among 230 unique proteins, 123 proteins were identified with higher confidence levels (at least two unique peptide sequences matched). Most of these proteins have been reported in plasma. However, there are 7 proteins, including JUP protein, suprabasin, annexin A2, transforming growth factor-beta-induced protein ig-h3 (βig-h3), V-set and immunoglobulin domain-containing protein 4 precursor, ifapsoriasin 2 and actin, cytoplasmic 1 have not been reported in serum.

Conclusions

Seven proteins may represent potential candidates of biomarkers. Annexin A2 is of special interest since it may play a role in the regulation of intercellular adhesion and cell proliferation.

Rodriguez-Panadero F, Borderas Naranjo F, Lopez Mejias J. Pleural metastatic tumours and effusions. Frequency and pathogenic mechanisms in a post-mortem series. Eur Respir J. 1989;2(4):366–9.PubMed

Medford AR, Maskell N. Pleural effusion. Postgrad Med J. 2005;81(961):702–10. doi:10.1136/pgmj.2005.035352.PubMedCrossRef

Chernow B, Sahn SA. Carcinomatous involvement of the pleura: an analysis of 96 patients. Am J Med. 1977;63(5):695–702.PubMedCrossRef

Tyan YC, Wu HY, Su WC, Chen PW, Liao PC. Proteomic analysis of human pleural effusion. Proteomics. 2005;5(4):1062–74. doi:10.1002/pmic.200401041.PubMedCrossRef

Adkins JN, Varnum SM, Auberry KJ, Moore RJ, Angell NH, Smith RD, et al. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry. Mol Cell Proteomics. 2002;1(12):947–55.PubMedCrossRef

Chignard N, Beretta L. Proteomics for hepatocellular carcinoma marker discovery. Gastroenterology. 2004;127(5 Suppl 1):S120–5.PubMedCrossRef

Davidson B, Espina V, Steinberg SM, Florenes VA, Liotta LA, Kristensen GB, et al. Proteomic analysis of malignant ovarian cancer effusions as a tool for biologic and prognostic profiling. Clin Cancer Res. 2006;12(3 Pt 1):791–9. doi:10.1158/1078-0432.CCR-05-2516.PubMedCrossRef

Bloomston M, Zhou JX, Rosemurgy AS, Frankel W, Muro-Cacho CA, Yeatman TJ. Fibrinogen gamma overexpression in pancreatic cancer identified by large-scale proteomic analysis of serum samples. Cancer Res. 2006;66(5):2592–9. doi:10.1158/0008-5472.CAN-05-3659.PubMedCrossRef

Tyan YC, Wu HY, Lai WW, Su WC, Liao PC. Proteomic profiling of human pleural effusion using two-dimensional nano liquid chromatography tandem mass spectrometry. J Proteome Res. 2005;4(4):1274–86. doi:10.1021/pr049746c.PubMedCrossRef

10.

Kersey PJ, Duarte J, Williams A, Karavidopoulou Y, Birney E, Apweiler R. The International Protein Index: an integrated database for proteomics experiments. Proteomics. 2004;4(7):1985–8. doi:10.1002/pmic.200300721.PubMedCrossRef

11.

Wu W, Tang X, Hu W, Lotan R, Hong WK, Mao L. Identification and validation of metastasis-associated proteins in head and neck cancer cell lines by two-dimensional electrophoresis and mass spectrometry. Clin Exp Metastasis. 2002;19(4):319–26.PubMedCrossRef

12.

Danielsen EM, van Deurs B, Hansen GH. “Nonclassical” secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane. Biochemistry. 2003;42(49):14670–6. doi:10.1021/bi0355239.PubMedCrossRef

13.

Farber SA, De Rose RA, Olson ES, Halpern ME. The zebrafish annexin gene family. Genome Res. 2003;13(6A):1082–96. doi:10.1101/gr.47960313/6a/1082

14.

MacLeod TJ, Kwon M, Filipenko NR, Waisman DM. Phospholipid-associated annexin A2–S100A10 heterotetramer and its subunits: characterization of the interaction with tissue plasminogen activator, plasminogen, and plasmin. J Biol Chem. 2003;278(28):25577–84. doi:10.1074/jbc.M301017200M301017200.PubMedCrossRef

15.

Xiong XD, Xu LY, Shen ZY, Cai WJ, Luo JM, Han YL, et al. Identification of differentially expressed proteins between human esophageal immortalized and carcinomatous cell lines by two-dimensional electrophoresis and MALDI-TOF-mass spectrometry. World J Gastroenterol. 2002;8(5):777–81.PubMed

16.

Li Cui XZ, Zhang XP, Chen ZC WX, Feng XP ZP, Li JL, Yi H, et al. Comparative analysis of serum proteomes for discovery of biomarkers for human lung squamous carcinoma. Chin J Biochem Mol Biol. 2005;3:357–68.

17.

Skonier J, Neubauer M, Madisen L, Bennett K, Plowman GD, Purchio AF. cDNA cloning and sequence analysis of beta ig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-beta. DNA Cell Biol. 1992;11(7):511–22.PubMedCrossRef

18.

Williams AF, Barclay AN. The immunoglobulin superfamily-domains for cell surface recognition. Ann Rev Immunol. 1988;6(1):381–405.CrossRef

19.

Wiesmann C, Katschke KJ, Yin J, Helmy KY, Steffek M, Fairbrother WJ, et al. Structure of C3b in complex with CRIg gives insights into regulation of complement activation. Nature. 2006;444(7116):217–20.PubMedCrossRef

20.

Schroeder J-M, Hansmann B, Wu Z. C-terminal ifapsoriasin fragments as antimicrobial peptides, the production thereof and use thereof. Google Patents; 2008.

21.

Wu Z. Analysis of two human gene clusters involved in innate immunity: dissertation; 2005.

22.

Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, et al. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004;14(10B):2121–7. doi:10.1101/gr.2596504.

23.

Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003;13(10):2265–70. doi:10.1101/gr.12930031293003.PubMedCrossRef

24.

Jindal HK, Chaney WG, Anderson CW, Davis RG, Vishwanatha JK. The protein-tyrosine kinase substrate, calpactin I heavy chain (p36), is part of the primer recognition protein complex that interacts with DNA polymerase alpha. J Biol Chem. 1991;266(8):5169–76.PubMed

25.

Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, et al. Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. Mol Cell Proteomics. 2005;4(9):1240–50. doi:10.1074/mcp.M500089-MCP200.

26.

Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, et al. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006;127(3):635–48. doi:10.1016/j.cell.2006.09.026.PubMedCrossRef

27.

Chi A, Valencia JC, Hu ZZ, Watabe H, Yamaguchi H, Mangini NJ, et al. Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J Proteome Res. 2006;5(11):3135–44. doi:10.1021/pr060363j.PubMedCrossRef

28.

Langnaese K, Colleaux L, Kloos DU, Fontes M, Wieacker P. Cloning of Z39Ig, a novel gene with immunoglobulin-like domains located on human chromosome X. Biochim Biophys Acta. 2000;1492(2–3):522–5. S0167-4781(00)00131-7 [pii].

29.

Wu Z. The human intermediate filament-associated protein family. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.

30.

Procaccio V, Salazar G, Ono S, Styers ML, Gearing M, Davila A, et al. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am J Hum Genet. 2006;78(6):947–60. doi:10.1086/504271.PubMedCentralPubMedCrossRef

Title: Proteomic analysis of pleural effusion from lung adenocarcinoma patients by shotgun strategy
Authors: Shu-hong Sheng
Hui-li Zhu
Publication date: 01-02-2014
Publisher: Springer Milan
Published in: Clinical and Translational Oncology / Issue 2/2014
Print ISSN: 1699-048X
Electronic ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-013-1054-9

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