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Indian Journal of Otolaryngology and Head & Neck Surgery

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01-08-2013 | Original Article

Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis

Authors: Aleksandar Perić, Danilo Vojvodić, Aneta V. Perić, Vesna Radulović, Olivera Miljanović

Published in: Indian Journal of Otolaryngology and Head & Neck Surgery | Special Issue 2/2013

Abstract

The aim of this study was to compare the cytokine levels in nasal fluid in subjects with nasal polyposis (NP) and co-morbid asthma and NP patients without asthma and to correlate these levels with clinical parameters of severity of disease. Forty NP patients (20 asthmatic and 20 nonasthmatic) were enrolled. Nasal secretion samples were collected from nasal cavities of all 40 subjects. The levels of Th1 cytokines IL-2, IL-12 and IFN-γ, Th2 cytokines IL-4, IL-5, IL-6, and IL-10, chemokine IL-8, and proinflammatory cytokines IL-1β, TNF-α and TNF-β were measured using flow cytometric method. Each of the 40 patients was staged clinically according to global nasal symptom score, endoscopic score, and Lund-Mackay computed tomography (CT) score. The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher (P < 0.01, P < 0.01, P < 0.05) in patients with NP and asthma compared with NP patients without asthma. Positive correlations were observed between concentration of IL-2 in nasal secretions and global nasal symptom score, endoscopic score, and Lund-Mackay score only in NP patients without asthma. We also found positive correlation between CT score and the levels of IL-8, IL-4, and IL-1β in nonasthmatic patients. Finally, our results showed a positive correlation between IL-5 levels in nasal fluid and endoscopic score only in asthmatic patients. NP in asthmatic patients have different immunological patterns compared to those without asthma. We also concluded that concentrations of cytokines measured in nasal fluid were not sensitive enough to determine the severity of disease.

Rinia AB, Kostamo K, Ebbens FA, van Drunen CM, Fokkens WJ (2007) Nasal polyposis: a cellular-based approach to answering questions. Allergy 62:348–358PubMedCrossRef

Fokkens W, Lund V (2007) European posistion paper on rhinosinusitis and nasal polyps. Rhinol Suppl 45(Suppl 20):1–136

Erbek SS, Yurtcu E, Erbek S, Atac FB, Sahin FI, Cakmak O (2007) Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis. Arch Otolaryngol Head Neck Surg 133:705–709PubMedCrossRef

Ramanathan M, Lee WK, Spannhake EW, Lane AP (2008) Th2 cytokines associated with chronic rhinosinusitis with polyps down-regulate the antimicrobial immune function of human sinonasal epithelial cells. Am J Rhinol 22:115–121PubMedCrossRef

Larsen K (1996) The clinical relationship of nasal polyps to asthma. Allergy Asthma Proc 17:243–249PubMedCrossRef

Král B, Krejsek J, Paráková Z, Kopecký O, Vokurková D, Derner V et al (1997) Some serum activity markers of airways inflammation in difficult-to-control asthma patients. Acta Medica (Hradec Králové) 40:61–70

Dunđerski J, Matić G (2009) Glucocorticoid receptor in health and disease. J Med Biochem 28:248–261

Jankowski R, Persoons M, Foliguet B, Coffinet L, Thomas C, Verient-Montaut B (2000) Eosinophil count in nasal secretions of subjects with and without nasal symptoms. Rhinology 38:23–32PubMed

Riechelmann H, Deutschle T, Rozsasi A, Keck T, Polzehl D, Bürner H (2005) Nasal biomarker profiles in acute and chronic rhinosinusitis. Clin Exp Allergy 35(9):1186–1191PubMedCrossRef

10.

Riechelmann H, Deutschle T, Friemel E, Gross HJ, Bachem M (2003) Biological markers in nasal secretions. Eur Respir J 21:600–605PubMedCrossRef

11.

Ohkubo K, Masakazu I, Pawankar R, Gotoh M, Yagi T, Okuda M (1998) Mechanisms of IL-6, IL-8 and GM-CSF release in nasal secretions of allergic patients after nasal challenge. Rhinology 36:156–161PubMed

12.

Bousquet J (2000) Global Initiative for Asthma (GINA) and its objectives. Clin Exp Allergy 30(Suppl 1):2–5PubMedCrossRef

13.

Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P et al (2004) Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. J Allergy Clin Immunol 113:462–469PubMedCrossRef

14.

Lildholdt T, Rundcrantz H, Bende M, Larsen K (1997) Glucocorticoid treatment for nasal polyps: the use of topical budesonide powder, intramuscular betamethasone, and surgical treatment. Arch Otolaryngol Head Neck Surg 123:595–600PubMedCrossRef

15.

Lund VJ, Mackay IS (1993) Staging in rhinosinusitis. Rhinology 31:183–184PubMed

16.

Watelet JB, Gevaert P, Holtappels G, Van Cauwenberge P, Bachert C (2004) Collection of nasal secretions for immunological analysis. Eur Arch Otorhinolaryngol 261:242–246PubMedCrossRef

17.

Bachert C, Van Kempen M, Van Cauwenberge P (1999) Regulation of proinflammatory cytokines in seasonal allergic rhinitis. Int Arch Allergy Immunol 118:375–379PubMedCrossRef

18.

Hens G, Hellings PW (2006) The nose: gatekeeper and trigger of bronchial disease. Rhinology 44:179–187PubMed

19.

Perić A, Gaćeša D (2008) Etiology and pathogenesis of chronic rhinosinusitis. Vojnosanit Pregl 65:699–702PubMedCrossRef

20.

Hamilos DL, Leung DJ, Wood R, Cunningham L, Bean DK, Yasruel Z et al (1995) Evidence for distinct cytokine expression in allergic versus nonallergic chronic sinusitis. J Allergy Clin Immunol 96:537–544PubMedCrossRef

21.

Bachert C, Wagenmann M, Rudack C, Höpken K, Hillebrandt M, Wang D et al (1998) The role of cytokines in infectious sinusitis and nasal polyposis. Allergy 53:2–13PubMedCrossRef

22.

Fan GK, Wang H, Takenaka H (2007) Eosinophil infiltration and activation in nasal polyposis. Acta Otolaryngol 127:521–526PubMedCrossRef

23.

Liu CM, Hong CY, Shun CT, Hsiao TY, Wang CC, Wang JS et al (2002) Inducible cyclooxygenase and interleukin-6 gene expression in nasal polyp fibroblasts. Arch Otolaryngol Head Neck Surg 128:945–951PubMedCrossRef

24.

Van Zele T, Gevaert P, Watelet JB, Claeys G, Holtappels G, Claeys C et al (2004) Staphylococcus aureus colonization and IgE antibody formation to enterotoxins is increased in nasal polyposis. J Allergy Clin Immunol 114:981–983PubMedCrossRef

25.

Hellings PW, Hens G, Meyts I, Bullens D, Vanoirbeek J, Gevaert P et al (2006) Aggravation of bronchial eosinophilia in mice by nasal and bronchial exposure to Staphylococcus aureus enterotoxin B. Clin Exp Allergy 36:1063–1071PubMedCrossRef

26.

Chen YS, Arab SF, Westhofen M, Lorenzen J (2005) Expression of interleukin-5, interleukin-8, and interleukin-10 mRNA in the ostiomeatal complex in nasal polyposis. Am J Rhinol 19:117–123PubMed

27.

Yoshifuku K, Matsune S, Ohori J, Sagara Y, Fukuiwa T, Kurono Y (2007) IL-4 and TNF-α increased the secretion of eotaxin from cultured fibroblasts of nasal polyps with eosinophil infiltration. Rhinology 45:235–241PubMed

28.

Saji F, Nonaka M, Pawankar R (2000) Expression of RANTES by IL-1β and TNF-α stimulated nasal polyp fibroblasts. Auris Nasus Larynx 27:247–252PubMedCrossRef

Title: Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis
Authors: Aleksandar Perić
Danilo Vojvodić
Aneta V. Perić
Vesna Radulović
Olivera Miljanović
Publication date: 01-08-2013
Publisher: Springer India
Published in: Indian Journal of Otolaryngology and Head & Neck Surgery / Issue Special Issue 2/2013
Print ISSN: 2231-3796
Electronic ISSN: 0973-7707
DOI: https://doi.org/10.1007/s12070-011-0447-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Correlation Between Cytokine Levels in Nasal Fluid and Scored Clinical Parameters in Patients with Nasal Polyposis

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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