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01-02-2018 | Original Article

Long-term presence of angiotensin II type 1 receptor autoantibody reduces aldosterone production by triggering Ca²⁺ overload in H295R cells

Authors: Jinghui Lei, Suli Zhang, Pengli Wang, Yang Liao, Jingwei Bian, Xiaochen Yin, Ye Wu, Lina Bai, Feng Wang, Xiaoli Yang, Huirong Liu

Published in: Immunologic Research | Issue 1/2018

Abstract

Preeclamptic women are reported to have inadequate plasma volume expansion coupled with a suppressed secretion of aldosterone; however, the specific mechanism of preeclampsia remains unclear. We demonstrated that the presence of long-term angiotensin II type 1 receptor autoantibody (AT1-AA) reduces aldosterone production by triggering a Ca²⁺ overload in H295R cells. AT1-AA was discovered in preeclamptic women and reported to activate AT₁R, and consequently elevate intracellular Ca²⁺. We found that AT1-AA significantly prolonged the time of intracellular Ca²⁺ elevation. Besides promoting aldosterone production as a second messenger, Ca²⁺ overload shows a cytotoxic effect. Our data reveals that long-term presence of AT1-AA triggered a Ca²⁺ overload and consequent impairment of aldosterone production, which could be prevented by a PKC inhibitor, Gö 6983, or a calcium channel inhibitor, nifedipine. These findings have clinical significance because AT₁R blockers are not recommended for treatment of preeclampsia due to their potential harm to the fetus. Our findings also emphasize a potential clinical benefit of immunoadsorption or neutralization of AT1-AA in preeclamptic women.

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Title: Long-term presence of angiotensin II type 1 receptor autoantibody reduces aldosterone production by triggering Ca2+ overload in H295R cells
Authors: Jinghui Lei
Suli Zhang
Pengli Wang
Yang Liao
Jingwei Bian
Xiaochen Yin
Ye Wu
Lina Bai
Feng Wang
Xiaoli Yang
Huirong Liu
Publication date: 01-02-2018
Publisher: Springer US
Published in: Immunologic Research / Issue 1/2018
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-017-8963-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Long-term presence of angiotensin II type 1 receptor autoantibody reduces aldosterone production by triggering Ca²⁺ overload in H295R cells

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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