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Immunologic Research
Published in: Immunologic Research 3/2015

01-07-2015

Practical NK cell phenotyping and variability in healthy adults

Authors: Laura S. Angelo, Pinaki P. Banerjee, Linda Monaco-Shawver, Joshua B. Rosen, George Makedonas, Lisa R. Forbes, Emily M. Mace, Jordan S. Orange

Published in: Immunologic Research | Issue 3/2015

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Abstract

Human natural killer (NK) cells display a wide array of surface and intracellular markers that indicate various states of differentiation and/or levels of effector function. These NK cell subsets exist simultaneously in peripheral blood and may vary among individuals. We examined variety among selected NK cell receptors expressed by NK cells from normal donors, as well as the distribution of select NK cell subsets and NK cell receptor expression over time in several individual donors. Peripheral blood mononuclear cells were evaluated using flow cytometry via fluorochrome-conjugated antibodies against a number of NK cell receptors. Results were analyzed for both mean fluorescence intensity (MFI) and the percent positive cells for each receptor. CD56bright and CD56dim NK cell subsets were also considered separately, as was variation in receptor expression in NK cell subsets over time in selected individuals. Through this effort, we provide ranges of NK cell surface receptor expression for a local adult population as well as provide insight into intra-individual variation.
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Metadata
Title
Practical NK cell phenotyping and variability in healthy adults
Authors
Laura S. Angelo
Pinaki P. Banerjee
Linda Monaco-Shawver
Joshua B. Rosen
George Makedonas
Lisa R. Forbes
Emily M. Mace
Jordan S. Orange
Publication date
01-07-2015
Publisher
Springer US
Published in
Immunologic Research / Issue 3/2015
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-015-8664-y

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