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Current Allergy and Asthma Reports

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Open Access 01-07-2020 | Allergens (RK Bush and S Vieths, Section Editors)

Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family

Authors: Lorenz Aglas, Wai Tuck Soh, Amin Kraiem, Mario Wenger, Hans Brandstetter, Fatima Ferreira

Published in: Current Allergy and Asthma Reports | Issue 7/2020

Abstract

Purpose of Review

Pathogenesis-related class 10 (PR-10) proteins are highly conserved plant proteins, which are induced in response to abiotic and biotic stress factors. To date, no unique biological function could be assigned to them. Rather a more general role of PR-10 in plant development and defense mechanisms has been proposed. In addition, some PR-10 proteins act as allergens by triggering allergic symptoms in sensitized individuals. Regardless of the diversity of reported activities, all PR-10 proteins share a common fold characterized by a solvent-accessible hydrophobic cavity, which serves as a binding site for a myriad of small-molecule ligands, mostly phytohormones and flavonoids.

Recent Findings

Most of available data relate to the ligand binding activity of allergenic PR-10, particularly for those belonging to Bet v 1 family of allergens. Bet v 1 and its homologues were shown to bind flavonoids with high affinity, but the specificity appears to differ between homologues from different species. The flavonoid Q3O-(Glc)-Gal was shown to specifically bind to hazelnut Cor a 1 but not to Bet v 1. Similarly, Q3OS bound only to the major isoform Bet v 1.0101 and not to other closely related isoforms. In contrast, Bet v 1 and hazelnut Cor a 1 showed very similar binding behavior towards other flavonoids such as quercetin, genistein, apigenin, daidzein, and resveratrol.

Summary

Recent research findings highlighted the importance of more precise knowledge of ligand binding for understanding the functional diversification of PR-10 proteins.

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Title: Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family
Authors: Lorenz Aglas
Wai Tuck Soh
Amin Kraiem
Mario Wenger
Hans Brandstetter
Fatima Ferreira
Publication date: 01-07-2020
Publisher: Springer US
Published in: Current Allergy and Asthma Reports / Issue 7/2020
Print ISSN: 1529-7322
Electronic ISSN: 1534-6315
DOI: https://doi.org/10.1007/s11882-020-00918-4

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Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family

Abstract

Purpose of Review

Recent Findings

Summary

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