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Obesity Surgery

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01-02-2019 | Original Contributions

Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition

Authors: Lee D. Ying, Gregory A. Breuer, Matthew O. Hubbard, Geoffrey S. Nadzam, John Hwa, Kathleen A. Martin

Published in: Obesity Surgery | Issue 2/2019

Abstract

Background

Sleeve gastrectomy with ileal transposition has been shown to be superior to sleeve gastrectomy alone for promoting weight loss in rat and porcine models. The absence of a mouse model for this procedure has impeded efforts to understand the molecular physiology underlying its efficacy. This study demonstrates the long-term survivability of sleeve gastrectomy with ileal transposition in mice.

Materials and Methods

In this study of technical feasibility, a sleeve gastrectomy with ileal transposition (SGIT), sleeve gastrectomy (SG), or sham surgery (SH) was performed on 7- to 8-week-old C57Bl/6J mice (n = 8 for each). To evaluate long-term survivability, mice were placed on an obesogenic diet and weighed weekly for 10 weeks. The intestinal identity of the transposed segment was assessed with gene expression analysis of duodenal-, jejunal-, and ileal-specific hormones using quantitative polymerase chain reaction.

Results

Overall, SGIT better prevented weight gain than the SG or sham procedures (10-week post-operative weight: SH 45.3 ± 1.0 g, SG 41.25 ± 1.6 g, SGIT 35.4 ± 0.8 g). Gene expression pattern analysis of three markers of intestinal identity (gastrin, cholecystokinin, and peptide YY) suggests that the ileal identity of the transposed segment is maintained 10 weeks after transposition.

Conclusions

We demonstrate for the first time a reproducible mouse model of sleeve gastrectomy with ileal transposition. Future studies utilizing this model will expand our understanding of the molecular pathways through which the hindgut regulates satiety.

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Title: Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition
Authors: Lee D. Ying
Gregory A. Breuer
Matthew O. Hubbard
Geoffrey S. Nadzam
John Hwa
Kathleen A. Martin
Publication date: 01-02-2019
Publisher: Springer US
Published in: Obesity Surgery / Issue 2/2019
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI: https://doi.org/10.1007/s11695-018-3555-7

At a glance: The STEP trials

Springer Medicine

Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition

Abstract

Background

Materials and Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Materials and Methods

Results

Conclusions

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