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Obesity Surgery
Published in: Obesity Surgery 2/2011

01-02-2011 | Physiology Research

Duodenal–Jejunal Bypass Surgery Does Not Increase Skeletal Muscle Insulin Signal Transduction or Glucose Disposal in Goto–Kakizaki Type 2 Diabetic Rats

Authors: Timothy P. Gavin, Ruben C. Sloan III, Eric Z. Lukosius, Melissa A. Reed, John R. Pender, Van Boghossian, Jacqueline J. Carter, Robert D. McKernie, Kushal Parikh, J. William Price, Edward B. Tapscott, Walter J. Pories, G. Lynis Dohm

Published in: Obesity Surgery | Issue 2/2011

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Abstract

Background

Duodenal–jejunal bypass (DJB) has been shown to reverse type 2 diabetes (T2DM) in Goto–Kakizaki (GK) rats, a rodent model of non-obese T2DM. Skeletal muscle insulin resistance is a hallmark decrement in T2DM. The aim of the current work was to investigate the effects of DJB on skeletal muscle insulin signal transduction and glucose disposal. It was hypothesized that DJB would increase skeletal muscle insulin signal transduction and glucose disposal in GK rats.

Methods

DJB was performed in GK rats. Sham operations were performed in GK and nondiabetic Wistar–Kyoto (WKY) rats. At 2 weeks post-DJB, oral glucose tolerance (OGTT) was measured. At 3 weeks post-DJB, insulin-induced signal transduction and glucose disposal were measured in skeletal muscle.

Results

In GK rats and compared to sham operation, DJB did not (1) improve fasting glucose or insulin, (2) improve OGTT, or (3) increase skeletal muscle insulin signal transduction or glucose disposal. Interestingly, skeletal muscle glucose disposal was similar between WKY-Sham, GK-Sham, and GK-DJB.

Conclusions

Bypassing of the proximal small intestine does not increase skeletal muscle glucose disposal. The lack of skeletal muscle insulin resistance in GK rats questions whether this animal model is adequate to investigate the etiology and treatments for T2DM. Additionally, bypassing of the foregut may lead to different findings in other animal models of T2DM as well as in T2DM patients.
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Metadata
Title
Duodenal–Jejunal Bypass Surgery Does Not Increase Skeletal Muscle Insulin Signal Transduction or Glucose Disposal in Goto–Kakizaki Type 2 Diabetic Rats
Authors
Timothy P. Gavin
Ruben C. Sloan III
Eric Z. Lukosius
Melissa A. Reed
John R. Pender
Van Boghossian
Jacqueline J. Carter
Robert D. McKernie
Kushal Parikh
J. William Price
Edward B. Tapscott
Walter J. Pories
G. Lynis Dohm
Publication date
01-02-2011
Publisher
Springer-Verlag
Published in
Obesity Surgery / Issue 2/2011
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-010-0304-y

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