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Journal of Gastrointestinal Surgery
Published in: Journal of Gastrointestinal Surgery 7/2015

01-07-2015 | Original Article

Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma

Authors: Karen Chiam, Tingting Wang, David I. Watson, George C. Mayne, Tanya S. Irvine, Tim Bright, Lorelle Smith, Imogen A. White, Joanne M. Bowen, Dorothy Keefe, Sarah K. Thompson, Michael E. Jones, Damian J. Hussey

Published in: Journal of Gastrointestinal Surgery | Issue 7/2015

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Abstract

Background

The poor prognosis and rising incidence of esophageal adenocarcinoma highlight the need for improved detection methods. The potential for circulating microRNAs (miRNAs) as biomarkers in other cancers has been shown, but circulating miRNAs have not been well characterized in esophageal adenocarcinoma. We investigated whether circulating exosomal miRNAs have potential to discriminate individuals with esophageal adenocarcinoma from healthy controls and non-dysplastic Barrett’s esophagus.

Methods

Seven hundred fifty-eight miRNAs were profiled in serum circulating exosomes from a cohort of 19 healthy controls, 10 individuals with Barrett’s esophagus, and 18 individuals with locally advanced esophageal adenocarcinoma. MiRNA expression was assessed using all possible permutations of miRNA ratios per individual. Four hundred eight miRNA ratios were differentially expressed in individuals with cancer compared to controls and Barrett’s esophagus (Mann-Whitney U test, P < 0.05). The 179/408 ratios discriminated esophageal adenocarcinoma from healthy controls and Barrett’s esophagus (linear regression, P < 0.05; area under receiver operating characteristic (ROC) > 0.7, P < 0.05). A multi-biomarker panel (RNU6-1/miR-16-5p, miR-25-3p/miR-320a, let-7e-5p/miR-15b-5p, miR-30a-5p/miR-324-5p, miR-17-5p/miR-194-5p) demonstrated enhanced specificity and sensitivity (area under ROC = 0.99, 95 % CI 0.96–1.0) over single miRNA ratios to distinguish esophageal adenocarcinoma from controls and Barrett’s esophagus.

Conclusions

This study highlights the potential for serum exosomal miRNAs as biomarkers for the detection of esophageal adenocarcinoma.
Appendix
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Metadata
Title
Circulating Serum Exosomal miRNAs As Potential Biomarkers for Esophageal Adenocarcinoma
Authors
Karen Chiam
Tingting Wang
David I. Watson
George C. Mayne
Tanya S. Irvine
Tim Bright
Lorelle Smith
Imogen A. White
Joanne M. Bowen
Dorothy Keefe
Sarah K. Thompson
Michael E. Jones
Damian J. Hussey
Publication date
01-07-2015
Publisher
Springer US
Published in
Journal of Gastrointestinal Surgery / Issue 7/2015
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI
https://doi.org/10.1007/s11605-015-2829-9

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