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Journal of Gastrointestinal Surgery

Published in:

01-02-2008 | original article

Metabolic Changes in the Pigliver During Warm Ischemia and Reperfusion Measured by Microdialysis

Authors: Anne-Sofie Kannerup, Peter Funch-Jensen, Henning Grønbæk, Rasmus Langelund Jørgensen, Frank Viborg Mortensen

Published in: Journal of Gastrointestinal Surgery | Issue 2/2008

Abstract

Aim

Portal triad clamping can cause ischemia–reperfusion injury. The aim of the study was to monitor metabolic changes by microdialysis before, during, and after warm ischemia in the pigliver.

Material and Methods

Eight pigs underwent laparotomy followed by ischemia by Pringle’s maneuver. One microdialysis catheter was placed in each of four liver lobes. A reference catheter was placed in a muscle. Microdialysis samples were collected at intervals of 30 min starting 2 h before 1 h of total ischemia followed by 3 h of reperfusion. Glucose, lactate, pyruvate, and glycerol concentrations were measured. Blood samples were drawn for determination of alanine aminotransferase, alkaline phosphatase, and bilirubin together with total leukocytes and prothrombin time.

Results

All parameters were stable during the baseline period. During the ischemic period, lactate levels increased significantly (P < 0.05) followed by a rapid decrease after reperfusion. A transient increase was observed for glucose and glycerol. Pyruvate showed a slight increase from the time of ischemia. The lactate–pyruvate ratio increased rapidly after initiating ischemia and decreased immediately after reperfusion. A slight increase in transaminase levels was observed.

Conclusions

During and after warm ischemia, there were profound metabolic changes in the pigliver observed with an increase in lactate, glucose, glycerol, and the lactate–pyruvate ratio. There were no differences between the four liver lobes, indicating the piglivers homogeneity.

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Title: Metabolic Changes in the Pigliver During Warm Ischemia and Reperfusion Measured by Microdialysis
Authors: Anne-Sofie Kannerup
Peter Funch-Jensen
Henning Grønbæk
Rasmus Langelund Jørgensen
Frank Viborg Mortensen
Publication date: 01-02-2008
Publisher: Springer-Verlag
Published in: Journal of Gastrointestinal Surgery / Issue 2/2008
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-007-0359-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Metabolic Changes in the Pigliver During Warm Ischemia and Reperfusion Measured by Microdialysis

Abstract

Aim

Material and Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Aim

Material and Methods

Results

Conclusions

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