Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Forensic Toxicology
Published in: Forensic Toxicology 2/2019

Open Access 01-07-2019 | Intoxication | Original Article

Comparison of measurement methods for carboxyhemoglobin in blood samples based on visible spectra with 17 institutions

Authors: Takeshi Ohmori, Yusuke Saito, Kou Mamiya, Sara Sasaoka, Yasuhito Suzuki, Yoshimasa Namekawa, Kazunori Otsuka, Seiichi Kogure, Akira Mochizuki, Yosuke Nomura, Kazuyo Asaoka, Takaoki Saito, Kazuyuki Yoshida, Masaki Ojima, Takako Koizumi, Mitsuru Kumihashi, Hiroshi Shimada, Seiya Wakita, Mai Otsuka, Yasuo Seto

Published in: Forensic Toxicology | Issue 2/2019

Login to get access

Abstract

Purpose

An inter-method variation experiment to evaluate visible spectrophotometric methods for determining carboxyhemoglobin (CO-Hb) in blood was performed in 17 different forensic science laboratories.

Methods

Three reference samples were prepared and the actual percentages of CO-Hb (%CO-Hb) in reference samples were determined with gas chromatography. The %CO-Hb values in reference samples were measured via four procedures: (1) the “Standard method of chemical analysis in poisoning 2017” (edited by the Pharmaceutical Society of Japan), (2) the isosbestic point method (developed by the Department of Forensic Medicine, Kagawa University), (3) the alkaline condition method, and (4) ISO 27368 with a slight modification.

Results

Procedures (1) and (2) gave results approximately the same as the actual values in low-%CO-Hb samples, but both methods underestimated %CO-Hb levels in blood samples with high %CO-Hb. Using a formula reported by Katsumata et al., procedure (3) overestimated %CO-Hb levels in blood samples with low %CO-Hb, while using the modified formula, gave underestimates in all samples. Procedure (4) gave %CO-Hb values relatively close to the actual values. Thus, an accuracy evaluation test was performed for this procedure and the values obtained were close to the actual CO-Hb content levels.

Conclusions

Procedure (4) was considered sufficiently accurate and thus recommendable for use in forensic science laboratories.
Literature
1.
National Research Institute of Police Science (2017) Annual case report of drug and toxic poisoning in Japan No. 60 (Jan-Dec 2016). National Research Institute of Police Science, Kashiwa (in Japanese)
2.
Yoshioka E, Hanley SJB, Kawanishi Y, Saijo Y (2014) Epidemic of charcoal burning suicide in Japan. Br J Psychiatry 204:274–282CrossRefPubMed
3.
National Research Institute of Police Science (2007) Annual case report of drug and toxic poisoning in Japan No. 49 (Jan-Dec 2005). National Research Institute of Police Science, Kashiwa (in Japanese)
4.
Raub JA, Mathieu-Nolf M, Hampson NB, Thom SR (2000) Carbon monoxide poisoning—a public health perspective. Toxicology 145:1–14CrossRefPubMed
5.
Prockop LD, Chichkova RI (2007) Carbon monoxide intoxication: an updated review. J Neurol Sci 262:122–130CrossRefPubMed
6.
Ishizawa F (1981) A study on the spectrophotometric determination of carboxyhemoglobin in blood—isosbestic point method. Jpn J Legal Med 35:191–200
7.
Rodkey FL, Hill TA, Pitts LL, Robertson RF (1979) Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood. Clin Chem 25:1388–1393PubMed
8.
Katsumata Y, Aoki M, Sato S, Suzuki O, Oya M, Yada S (1982) A simple spectrophotometry for determination of carbonhemoglobin in blood. J Forensic Sci 27:928–934PubMed
9.
International Organization for Standardization (2008) ISO27368: analysis of blood for asphyxiant toxicants—carbon monoxide and hydrogen cyanide. International Organization for Standardization, Geneva, pp 14–15
10.
Winek CL, Prex DM (1981) A comparative study of analytical methods to determine postmortem changes in carbon monoxide concentration. Forensic Sci Int 18:181–197CrossRefPubMed
11.
Sakata M, Haga M (1980) Rapid determination of carboxyhemoglobin by absorbance difference between double wave length. Jpn J Toxicol Sci 5:113–121CrossRef
12.
The Pharmaceutical Society of Japan (2017) Standard method of chemical analysis in poisoning 2017. Tokyo Kagaku Dojin, Tokyo, pp 40–45 (in Japanese)
13.
Tanaka N, Ameno K, Mostofa J, Ohkubo E, Kumihashi M, Kinoshita H (2010) Application of oximeter AVOX 4000 for the determination of CO-Hb in the forensic practice. Res Pract Forensic Med 53:39–43 (in Japanese)
14.
Lee C, Tam JCN, Kung L-K, Yim L (2003) Validity of CO-oximetric determination of carboxyhaemoglobin in putrefying blood and body cavity fluid. Forensic Sci Int 132:153–156CrossRefPubMed
15.
Ohmori T, Kumihashi M, Endo K, Nakazawa H, Takekawa K, Uchikawa T, Seto Y (2018) Comparison of visible spectrophotometric methods for measuring carboxyhemoglobin in blood sample. Jpn J Forensic Sci Technol 23:91–101 (in Japanese with English abstract) CrossRef
16.
Ohmori T, Otsuka M, Seto Y (2017) Measurement of carbon monoxide in blood sample using gas-chromatography with barrier discharge. Rep Natl Res Inst Police Sci 66:59–64 (in Japanese with English abstract)
17.
Seto Y, Kataoka M, Tsuge K (2001) Stability of blood carbon monoxide and hemoglobin during heating. Forensic Sci Int 121:144–150CrossRefPubMed
18.
Taylor JD, Miller JDM (1965) A source of error in the cyanmetheglobin method of determination of hemoglobin concentration in blood containing carbon monoxide. Am J Clin Pathol 43:265–271CrossRefPubMed
19.
20.
21.
22.
Van Kampen EJ, Zijlstra WG (1966) Determination of hemoglobin and its derivatives. Adv Clin Chem 8:141–187CrossRef
Metadata
Title
Comparison of measurement methods for carboxyhemoglobin in blood samples based on visible spectra with 17 institutions
Authors
Takeshi Ohmori
Yusuke Saito
Kou Mamiya
Sara Sasaoka
Yasuhito Suzuki
Yoshimasa Namekawa
Kazunori Otsuka
Seiichi Kogure
Akira Mochizuki
Yosuke Nomura
Kazuyo Asaoka
Takaoki Saito
Kazuyuki Yoshida
Masaki Ojima
Takako Koizumi
Mitsuru Kumihashi
Hiroshi Shimada
Seiya Wakita
Mai Otsuka
Yasuo Seto
Publication date
01-07-2019
Publisher
Springer Singapore
Keyword
Intoxication
Published in
Forensic Toxicology / Issue 2/2019
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI
https://doi.org/10.1007/s11419-019-00469-y

Other articles of this Issue 2/2019

Forensic Toxicology 2/2019 Go to the issue

Case Report

Determination of ocfentanil and W-18 in a suspicious heroin-like powder in Belgium

Original Article

Hyperreflexia induced by XLR-11 smoke is caused by the pyrolytic degradant

Original Article

Identification and structural characterization of synthetic cathinones: N-propylcathinone, 2,4-dimethylmethcathinone, 2,4-dimethylethcathinone, 2,4-dimethyl-α-pyrrolidinopropiophenone, 4-bromo-α-pyrrolidinopropiophenone, 1-(2,3-dihydro-1H-inden-5-yl)-2-(pyrrolidin-1-yl)hexan-1-one and 2,4-dimethylisocathinone

Short Communication

A rapid screening color test for glyphosate using dabsyl derivatization

Letter to the Editor

Synovial fluid as an alternative specimen for quantification of drugs of abuse by GC–MS

Original Article

Cytotoxicity of the synthetic cannabinoids 5C-AKB48, 5F-MDMB-PINACA, ADB-CHMINACA, MDMB-CHMICA and NM-2201 in A549 and TR146 cell lines