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01-09-2018 | Original Paper

Hydroxyobtustyrene protects neuronal cells from chemical hypoxia-induced cell death

Authors: Takashi Iwai, Kengo Obara, Chihiro Ito, Hiroshi Furukawa, Jun-Ichiro Oka

Published in: Journal of Natural Medicines | Issue 4/2018

Abstract

Hydroxyobtustyrene is a derivative of cinnamyl phenol isolated from Dalbergia odorifera T. Chen. The heartwood, known as ‘JiangXiang’, is a traditional Chinese medicine. Previous studies showed that hydroxyobtustyrene inhibited the biosynthesis of prostaglandins, which are mediators of neuronal cell death in ischemia. However, it currently remains unclear whether hydroxyobtustyrene protects neurons against ischemic stress. In the present study, we investigated the protective effects of hydroxyobtustyrene against sodium cyanide (NaCN)-induced chemical ischemia. Hippocampal neurons were cultured from the cerebral cortices of E18 Wistar rats. The effects of hydroxyobtustyrene on neuronal survival and trophic effects were estimated under lower and higher cell density conditions. After the treatment of 1 mM NaCN with or without hydroxyobtustyrene, an MTT assay, Hoechst staining, and immunocytochemistry for cyclooxygenase (COX)-2 were performed. Hydroxyobtustyrene increased cell viability under lower, but not normal density conditions. Neither the neurite number nor the length was influenced by hydroxyobtustyrene. NaCN significantly decreased viability and increased fragmentation in cell nuclei, and these changes were prevented by hydroxyobtustyrene. Moreover, NaCN increased the number of COX-2-positive neurons, and this was significantly prevented by the co-treatment with hydroxyobtustyrene. Therefore, hydroxyobtustyrene protected cultured hippocampal neurons against NaCN-induced chemical ischemia, which may be mediated by the inhibition of COX-2 production.

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Title: Hydroxyobtustyrene protects neuronal cells from chemical hypoxia-induced cell death
Authors: Takashi Iwai
Kengo Obara
Chihiro Ito
Hiroshi Furukawa
Jun-Ichiro Oka
Publication date: 01-09-2018
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 4/2018
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-1224-8

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Hydroxyobtustyrene protects neuronal cells from chemical hypoxia-induced cell death

Abstract

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