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Published in: Journal of Natural Medicines 3/2013

01-07-2013 | Note

Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix

Authors: Rie Tanaka, Marina Yamazaki, Keiko Hasada, Akito Nagatsu

Published in: Journal of Natural Medicines | Issue 3/2013

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Abstract

A quantitative 1H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45 %. We demonstrated that this method is useful for quantitative analysis of crude drugs.
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Metadata
Title
Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix
Authors
Rie Tanaka
Marina Yamazaki
Keiko Hasada
Akito Nagatsu
Publication date
01-07-2013
Publisher
Springer Japan
Published in
Journal of Natural Medicines / Issue 3/2013
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-012-0711-6

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