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01-07-2011 | Note

Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

Authors: Hao Hong, Hu-Biao Chen, Dong-Hui Yang, Ming-Ying Shang, Xuan Wang, Shao-Qing Cai, Masayuki Mikage

Published in: Journal of Natural Medicines | Issue 3-4/2011

Abstract

In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine:water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.

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Title: Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography
Authors: Hao Hong
Hu-Biao Chen
Dong-Hui Yang
Ming-Ying Shang
Xuan Wang
Shao-Qing Cai
Masayuki Mikage
Publication date: 01-07-2011
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 3-4/2011
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-011-0528-8

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Comparison of contents of five ephedrine alkaloids in three official origins of Ephedra Herb in China by high-performance liquid chromatography

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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