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01-04-2006 | Note

DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

Authors: Yahong Guo, Kenji Kondo, Susumu Terabayashi, Yutaka Yamamoto, Hiroshi Shimada, Masao Fujita, Takeshi Kawasaki, Takuro Maruyama, Yukihiro Goda, Hajime Mizukami

Published in: Journal of Natural Medicines | Issue 2/2006

Abstract

The internal transcribed spacer 1 (ITS1) region between 18S and 5.8S rDNA was amplified from 25 crude drug specimens of So-jutsu (Atractylodes lancea rhizome) and 11 specimens of Byaku-jutsu (Atractylodes rhizome) obtained in the Chinese market. Based on the comparison of the nucleotide sequences of the PCR products with those determined in five Atractylodes species, we were able to identify the plant origins of these crude drugs. Of the 25 specimens of So-jutsu analyzed, 20 were identified to be derived from either A. chinensis, A. lancea or the interspecific hybrid between these two species, whereas five specimens were identified to be derived from A. japonica, i.e. Byaku-jutsu. In contrast, all of the specimens of Byaku-jutsu analyzed were derived from either A. ovata or A. japonica. A PCR-based method was established for the rapid identification and/or discrimination of So-jutsu and Byaku-jutsu without sequencing.

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Title: DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region
Authors: Yahong Guo
Kenji Kondo
Susumu Terabayashi
Yutaka Yamamoto
Hiroshi Shimada
Masao Fujita
Takeshi Kawasaki
Takuro Maruyama
Yukihiro Goda
Hajime Mizukami
Publication date: 01-04-2006
Publisher: Springer-Verlag
Published in: Journal of Natural Medicines / Issue 2/2006
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-006-0032-8

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DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

Abstract

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