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01-08-2020 | Positron Emission Tomography | Brief Article

Type of Anaesthetic Influences [¹¹C]MDL100,907 Binding to 5HT_2A Receptors in Porcine Brain

Authors: Anne M. Landau, Ove Noer, Aage Kristian Olsen Alstrup, Hélène Audrain, Gregers Wegener, Albert Gjedde, Doris J. Doudet, Michael Winterdahl

Published in: Molecular Imaging and Biology | Issue 4/2020

Abstract

Purpose

Anaesthesia routinely is used in animal neuroimaging in order to reduce head motion artefacts and minimize the influence of stress. However, anaesthetics can modify radioligand binding profiles at receptor targets studied by positron emission tomography (PET). Here, we determined the effects of two routine anaesthetics on the binding of a tracer of the serotonin 5HT_2A receptors.

Procedures

Isoflurane- and propofol-anesthetised Göttingen minipigs were imaged with [¹¹C]MDL100,907 PET and analysed using regions of interest and statistical non-parametric mapping.

Results

The binding potentials of the tracer in striatum under isoflurane anaesthesia significantly exceeded those obtained under propofol anaesthesia, an effect we attribute to the higher blood flow in brain induced by the former.

Conclusions

Interactions between radioligands and anaesthesia must be carefully evaluated in the design of in vivo neuroimaging and interpretation of data.

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Title: Type of Anaesthetic Influences [11C]MDL100,907 Binding to 5HT2A Receptors in Porcine Brain
Authors: Anne M. Landau
Ove Noer
Aage Kristian Olsen Alstrup
Hélène Audrain
Gregers Wegener
Albert Gjedde
Doris J. Doudet
Michael Winterdahl
Publication date: 01-08-2020
Publisher: Springer International Publishing
Keywords: Positron Emission Tomography
Isoflurane
Propofol
Published in: Molecular Imaging and Biology / Issue 4/2020
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-020-01476-x

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Type of Anaesthetic Influences [¹¹C]MDL100,907 Binding to 5HT_2A Receptors in Porcine Brain

Abstract

Purpose

Procedures

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

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Abstract

Purpose

Procedures

Results

Conclusions

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