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Open Access 01-06-2018 | Research Article

PET Imaging with S-[¹¹C]Methyl-L-Cysteine and L-[Methyl-¹¹C]Methionine in Rat Models of Glioma, Glioma Radiotherapy, and Neuroinflammation

Authors: Andrea Parente, Aren van Waarde, Alexandre Shoji, Daniele de Paula Faria, Bram Maas, Rolf Zijlma, Rudi A. J. O. Dierckx, Johannes A. Langendijk, Erik F.J. de Vries, Janine Doorduin

Published in: Molecular Imaging and Biology | Issue 3/2018

Abstract

Purpose

S-[¹¹C]-methyl-L-cysteine ([¹¹C]MCYS) has been claimed to offer higher tumor selectivity than L-[methyl- ¹¹C]methionine ([¹¹C]MET). We examined this claim in animal models.

Procedures

Rats with implanted untreated (n = 10) or irradiated (n = 7, 1 × 25 Gy, on day 8) orthotopic gliomas were scanned after 6, 9, and 12 days, using positron emission tomography. Rats with striatal injections of saline (n = 9) or bacterial lipopolysaccharide (n = 9) were scanned after 3 days.

Results

Uptake of the two tracers in untreated gliomas was similar. [¹¹C]MCYS was not accumulated in salivary glands, nasal epithelium, and healing wounds, in contrast to [¹¹C]MET, but showed 40 % higher accumulation in the healthy brain. Both tracers showed a reduced tumor uptake 4 days after irradiation and minor accumulation in inflamed striatum. [¹¹C]MCYS indicated higher lesion volumes than [¹¹C]MET (untreated tumor + 47 %; irradiated tumor up to + 500 %; LPS-inflamed striatum + 240 %).

Conclusions

[¹¹C]MCYS was less accumulated in some non-tumor tissues than [¹¹C]MET, but showed lower tumor-to-brain contrast.

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Title: PET Imaging with S-[11C]Methyl-L-Cysteine and L-[Methyl-11C]Methionine in Rat Models of Glioma, Glioma Radiotherapy, and Neuroinflammation
Authors: Andrea Parente
Aren van Waarde
Alexandre Shoji
Daniele de Paula Faria
Bram Maas
Rolf Zijlma
Rudi A. J. O. Dierckx
Johannes A. Langendijk
Erik F.J. de Vries
Janine Doorduin
Publication date: 01-06-2018
Publisher: Springer International Publishing
Published in: Molecular Imaging and Biology / Issue 3/2018
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-017-1137-z

At a glance: The STEP trials

Springer Medicine

PET Imaging with S-[¹¹C]Methyl-L-Cysteine and L-[Methyl-¹¹C]Methionine in Rat Models of Glioma, Glioma Radiotherapy, and Neuroinflammation

Abstract

Purpose

Procedures

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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