Distribution of Matrix Metalloproteinases in Human Atherosclerotic Carotid Plaques and Their Production by Smooth Muscle Cells and Macrophage Subsets | springermedicine.com

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Molecular Imaging and Biology

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Open Access 01-04-2016 | Research Article

Distribution of Matrix Metalloproteinases in Human Atherosclerotic Carotid Plaques and Their Production by Smooth Muscle Cells and Macrophage Subsets

Authors: Nynke A. Jager, Bastiaan M. Wallis de Vries, Jan-Luuk Hillebrands, Niels J. Harlaar, René A. Tio, Riemer H. J. A. Slart, Gooitzen M. van Dam, Hendrikus H. Boersma, Clark J. Zeebregts, Johanna Westra

Published in: Molecular Imaging and Biology | Issue 2/2016

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Abstract

Purpose

In this study, the potential of matrix metalloproteinase (MMP) sense for detection of atherosclerotic plaque instability was explored. Secondly, expression of MMPs by macrophage subtypes and smooth muscle cells (SMCs) was investigated.

Procedures

Twenty-three consecutive plaques removed during carotid endarterectomy were incubated in MMPSense™ 680 and imaged with IVIS® Spectrum. mRNA levels of MMPs, macrophage markers, and SMCs were determined in plaque specimens, and in in vitro differentiated M1 and M2 macrophages.

Results

There was a significant difference between autofluorescence signals and MMPSense signals, both on the intraluminal and extraluminal sides of plaques. MMP-9 and CD68 messenger RNA (mRNA) expression was higher in hot spots, whereas MMP-2 and αSMA expression was higher in cold spots. In vitro M2 macrophages had higher mRNA expression of MMP-1, MMP-9, MMP-12, and TIMP-1 compared to M1 macrophages.

Conclusion

MMP-9 is most dominantly MMP present in atherosclerotic plaques and is produced by M2 rather than M1 macrophages.

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Title: Distribution of Matrix Metalloproteinases in Human Atherosclerotic Carotid Plaques and Their Production by Smooth Muscle Cells and Macrophage Subsets
Authors: Nynke A. Jager
Bastiaan M. Wallis de Vries
Jan-Luuk Hillebrands
Niels J. Harlaar
René A. Tio
Riemer H. J. A. Slart
Gooitzen M. van Dam
Hendrikus H. Boersma
Clark J. Zeebregts
Johanna Westra
Publication date: 01-04-2016
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 2/2016
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-015-0882-0

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