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01-02-2016 | Laboratory Investigation

XBP1 silencing decreases glioma cell viability and glycolysis possibly by inhibiting HK2 expression

Authors: Yaohua Liu, Xu Hou, Min Liu, Zhuowen Yang, Yunke Bi, Huichao Zou, Jianing Wu, Hui Che, Chenguang Li, Xiaoxiong Wang, Kaikai Wang, Chen Zhong, Jiakang Zhang, Tao Yu, Qilong Bian, Shuang Chai, Huailei Liu, Jing Ai, Shiguang Zhao

Published in: Journal of Neuro-Oncology | Issue 3/2016

Abstract

Glioma cells rely on glycolysis to obtain energy and sustain their survival under microenvironmental stress in vivo. The mechanisms of regulation of glycolysis in glioma cells are unclear. Signaling pathway mediated by the transcription factor X box-binding protein 1 (XBP1) is one of the most important pathways of unfolded protein response which is comprehensively activated in cancer cells upon the microenvironmental stress. Here we showed that XBP1 was significantly activated in glioma tissues in vivo. XBP1 silencing resulted in decreasing of glioma cell viability and ATP/lactate production under hypoxia, which is possibly mediated by inhibition of Hexokinase II (HK2)’s expression. More importantly, XBP1 silenced glioma cells showed the decrease of tumor formation capacity. Our results revealed that XBP1s activation was involved in glioma glycolysis regulation and might be a potential molecular target for glioma treatment.

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Title: XBP1 silencing decreases glioma cell viability and glycolysis possibly by inhibiting HK2 expression
Authors: Yaohua Liu
Xu Hou
Min Liu
Zhuowen Yang
Yunke Bi
Huichao Zou
Jianing Wu
Hui Che
Chenguang Li
Xiaoxiong Wang
Kaikai Wang
Chen Zhong
Jiakang Zhang
Tao Yu
Qilong Bian
Shuang Chai
Huailei Liu
Jing Ai
Shiguang Zhao
Publication date: 01-02-2016
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 3/2016
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-015-2003-y

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XBP1 silencing decreases glioma cell viability and glycolysis possibly by inhibiting HK2 expression

Abstract

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Springer Medicine

Abstract

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