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Published in: Journal of Neuro-Oncology 1/2011

01-08-2011 | Laboratory Investigation - Human/Animal Tissue

NGAL and NGALR are frequently overexpressed in human gliomas and are associated with clinical prognosis

Authors: Ming-Fa Liu, Tao Jin, Jin-Hui Shen, Zhong-Ying Shen, Zhi-Chao Zheng, Zeng-Liang Zhang, Li-Yan Xu, En-Min Li, Hai-Xiong Xu

Published in: Journal of Neuro-Oncology | Issue 1/2011

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Abstract

Recently, neutrophil gelatinase-associated lipocalin (NGAL) and its cell surface receptor, NGALR, have been shown to have critical roles in the biology of various tumors. Therefore, we investigated the expression of NGAL and NGALR in tumor sections obtained from patients with gliomas, and compared these results with the clinical characteristics of the patients. Using immunohistochemical assays, the expression levels of NGAL and NGALR were found to be up-regulated in tumor tissues, and to be related to tumor grade (p < 0.001). A positive correlation between expression of the two markers was also observed in these assays (r = 0.849; p < 0.001). Overexpression of NGAL and NGALR in glioma tissues was also confirmed in western blot analysis and real-time quantitative RT-PCR assays. Furthermore, overexpression of NGAL and NGALR was found to be significantly associated with poor prognosis (p < 0.001 in each case). Multivariate analysis identified patient age, tumor grade, and expression levels of NGAL and NGALR to be independent prognostic factors. In particular, NGAL(2+)/NGALR(2+) tissues were associated with lower rates of survival (risk ratio, 1.378; 95% CI, 1.102–1.724; p = 0.005). These findings suggest that NGAL and NGALR expression are frequently up-regulated in gliomas, and are closely associated with poor clinical outcome.
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Metadata
Title
NGAL and NGALR are frequently overexpressed in human gliomas and are associated with clinical prognosis
Authors
Ming-Fa Liu
Tao Jin
Jin-Hui Shen
Zhong-Ying Shen
Zhi-Chao Zheng
Zeng-Liang Zhang
Li-Yan Xu
En-Min Li
Hai-Xiong Xu
Publication date
01-08-2011
Publisher
Springer US
Published in
Journal of Neuro-Oncology / Issue 1/2011
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI
https://doi.org/10.1007/s11060-010-0486-0

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