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01-12-2018 | Original Article

Gradual common carotid artery occlusion as a novel model for cerebrovascular Hypoperfusion

Authors: Dominic D. Quintana, Xuefang Ren, Heng Hu, Elizabeth B. Engler-Chiurazzi, Stephanie L. Rellick, Sara E. Lewis, Jessica M. Povroznik, James W. Simpkins, Mohammad Alvi

Published in: Metabolic Brain Disease | Issue 6/2018

Abstract

Chronic cerebrovascular hypoperfusion results in vascular dementia and increases predisposition to lacunar infarcts. However, there are no suitable animal models. In this study, we developed a novel model for chronic irreversible cerebral hypoperfusion in mice. Briefly, an ameroid constrictor was placed on the right carotid artery to gradually occlude the vessel, while a microcoil was placed on the left carotid artery to prevent compensation of the blood flow. This procedure resulted in a gradual hypoperfusion developing over a period of 34 days with no cerebral blood flow recovery. Histological analysis of the brain revealed neuronal and axonal degeneration as well as necrotic lesions. The most severely affected regions were located in the hippocampus and the corpus callosum. Overall, our paradigm is a viable model to study brain pathology resulting from gradual cerebrovascular hypoperfusion.

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Title: Gradual common carotid artery occlusion as a novel model for cerebrovascular Hypoperfusion
Authors: Dominic D. Quintana
Xuefang Ren
Heng Hu
Elizabeth B. Engler-Chiurazzi
Stephanie L. Rellick
Sara E. Lewis
Jessica M. Povroznik
James W. Simpkins
Mohammad Alvi
Publication date: 01-12-2018
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 6/2018
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-018-0312-5

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