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01-09-2014 | Research Article

Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats

Authors: Vahid Ghazizadeh, Mustafa Nazıroğlu

Published in: Metabolic Brain Disease | Issue 3/2014

Abstract

Incidence rates of epilepsy and use of Wi-Fi worldwide have been increasing. TRPV1 is a Ca²⁺ permeable and non-selective channel, gated by noxious heat, oxidative stress and capsaicin (CAP). The hyperthermia and oxidant effects of Wi-Fi may induce apoptosis and Ca²⁺ entry through activation of TRPV1 channel in epilepsy. Therefore, we tested the effects of Wi-Fi (2.45 GHz) exposure on Ca²⁺ influx, oxidative stress and apoptosis through TRPV1 channel in the murine dorsal root ganglion (DRG) and hippocampus of pentylentetrazol (PTZ)-induced epileptic rats. Rats in the present study were divided into two groups as controls and PTZ. The PTZ groups were divided into two subgroups namely PTZ + Wi-Fi and PTZ + Wi-Fi + capsazepine (CPZ). The hippocampal and DRG neurons were freshly isolated from the rats. The DRG and hippocampus in PTZ + Wi-Fi and PTZ + Wi-Fi + CPZ groups were exposed to Wi-Fi for 1 hour before CAP stimulation. The cytosolic free Ca²⁺, reactive oxygen species production, apoptosis, mitochondrial membrane depolarization, caspase-3 and −9 values in hippocampus were higher in the PTZ group than in the control although cell viability values decreased. The Wi-Fi exposure induced additional effects on the cytosolic Ca²⁺ increase. However, pretreatment of the neurons with CPZ, results in a protection against epilepsy-induced Ca²⁺ influx, apoptosis and oxidative damages. In results of whole cell patch-clamp experiments, treatment of DRG with Ca²⁺ channel antagonists [thapsigargin, verapamil + diltiazem, 2-APB, MK-801] indicated that Wi-Fi exposure induced Ca²⁺ influx via the TRPV1 channels. In conclusion, epilepsy and Wi-Fi in our experimental model is involved in Ca²⁺ influx and oxidative stress-induced hippocampal and DRG death through activation of TRPV1 channels, and negative modulation of this channel activity by CPZ pretreatment may account for the neuroprotective activity against oxidative stress.

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Title: Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats
Authors: Vahid Ghazizadeh
Mustafa Nazıroğlu
Publication date: 01-09-2014
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 3/2014
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-014-9549-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Electromagnetic radiation (Wi-Fi) and epilepsy induce calcium entry and apoptosis through activation of TRPV1 channel in hippocampus and dorsal root ganglion of rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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