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Journal of Assisted Reproduction and Genetics

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01-03-2019 | Reproductive Physiology and Disease

Lipophilic statins inhibit growth and reduce invasiveness of human endometrial stromal cells

Authors: Anna Sokalska, Amanda B. Hawkins, Toshia Yamaguchi, Antoni J. Duleba

Published in: Journal of Assisted Reproduction and Genetics | Issue 3/2019

Abstract

Purpose

To compare effects of lipid-soluble statins (simvastatin, lovastatin, atorvastatin) and water-soluble statin (pravastatin) on growth and invasiveness of human endometrial stromal (HES) cells.

Methods

Endometrial biopsies were collected during the proliferative phase from five volunteers. HES cells were isolated and cultured in the absence or in the presence of simvastatin, lovastatin, atorvastatin, and pravastatin. Effects of statins on DNA synthesis, cell viability, activity of caspases 3/7 and invasiveness were evaluated.

Results

The proliferation of HES cells was significantly decreased by simvastatin (by 47–89%), lovastatin (by 46–78%), and atorvastatin (by 21–48%) in a concentration-dependent manner. Activity of executioner caspases 3/7 was significantly increased by simvastatin (by 10–25%), lovastatin (by 19%) and atorvastatin (by 7–10%) in a concentration-dependent manner. The greatest effects were observed in response to simvastatin. Accounting for the effects of statins on cell number, the invasiveness of HES cells was significantly decreased in cells treated with simvastatin (by 49%), lovastatin (by 54%), and atorvastatin (by 53%). Pravastatin had little or no effects on any of the tested endpoints.

Conclusions

Present findings demonstrate that only lipid-soluble among tested statins were effective in inhibition of growth and invasiveness of HES cells. These findings may have clinical relevance in treatment of endometriosis.

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Title: Lipophilic statins inhibit growth and reduce invasiveness of human endometrial stromal cells
Authors: Anna Sokalska
Amanda B. Hawkins
Toshia Yamaguchi
Antoni J. Duleba
Publication date: 01-03-2019
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 3/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-018-1352-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Lipophilic statins inhibit growth and reduce invasiveness of human endometrial stromal cells

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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