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Journal of Assisted Reproduction and Genetics

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01-02-2018 | Assisted Reproduction Technologies

Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application

Authors: Xiaopeng Zhao, Binbin Ma, Shaokang Mo, Lu Ma, Fei Chang, Liyuan Zhang, Fang Xu, Ling Wang

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2018

Abstract

Purpose

The purpose of this study is to investigate the application value of the extended embryo culture for 7–8 h in day 3 morning during IVF-ET process.

Methods

Embryos were retrospectively assessed during 08:00–09:00 on the morning of day 3 in the control group, and were assessed once again at 16:00 in the afternoon in the extended culture (EC) group. The embryos with good developmental potential were preferentially selected to transfer. The cumulative pregnancy outcomes were analyzed in one oocyte retrieval cycle.

Results

Similar proportions were found in the rates of cumulative clinical pregnancy, cumulative live birth, and the perinatal/neonatal outcomes per oocyte retrieval cycle (P > 0.05). But higher total clinical pregnancy rate, higher total implantation rate, and lower total abortion rate were obtained in the EC group (P < 0.05). After EC, 53.58% of the embryos were able to continue to develop. The transferred embryos were mainly composed of ≥ 8-cell embryos (75.90%) in the EC group and ≤ 8-cell embryos (82.92%) in the control group. Interestingly, the implantation rates were increasingly improved with the increasing blastomere number up to 56.31% at the morula stage in the EC group, while they were limited to 32.33% at 8-cell stage in the control group.

Conclusions

The extended culture of day 3 embryos for 7–8 h not only reduced the risk of IVF-ET treatment compared to blastocyst culture through another 2–3 days, but also improved the clinical outcomes and the efficiency of every transferred cycle and every transferred embryo.

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Title: Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application
Authors: Xiaopeng Zhao
Binbin Ma
Shaokang Mo
Lu Ma
Fei Chang
Liyuan Zhang
Fang Xu
Ling Wang
Publication date: 01-02-2018
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2018
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-017-1065-5

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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