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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 4/2016

01-04-2016 | Embryo Biology

Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems

Authors: Ivan Bochev, Kalina Belemezova, Atanas Shterev, Stanimir Kyurkchiev

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2016

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Abstract

Purpose

Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates.

Methods

Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA.

Results

Embryo co-culture with frozen ESCs resulted in a much lower pregnancy rate compared to the alternative system using fresh ESCs. Furthermore, cultivated frozen ESCs showed considerably decreased release of prolactin, IGFBP-1, and IL-6 compared to fresh ESCs, indicating that cryopreservation negatively affects their decidualization potential and cytokine production.

Conclusions

Altogether, this data illustrates the need for optimization and improvement of the existing autologous endometrial co-culture systems.
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Metadata
Title
Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems
Authors
Ivan Bochev
Kalina Belemezova
Atanas Shterev
Stanimir Kyurkchiev
Publication date
01-04-2016
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 4/2016
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-016-0651-2

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