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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 6/2015

01-06-2015 | Gamete Biology

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia

Authors: Mahmoud Hashemitabar, Susan Sabbagh, Mahmoud Orazizadeh, Atta Ghadiri, Maryam Bahmanzadeh

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2015

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Abstract

Purpose

Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients.

Methods

Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry.

Results

Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p < 0.05) were excised and exploited using MALDI-TOF-TOF mass spectrometry. They led to the identification of the following 14 unique proteins: Tubulin beta 2B; glutathione S-transferase Mu 3; keratin, type II cytoskeletal 1; outer dense fiber protein 2; voltage-dependent anion-selective channel protein 2; A-kinase anchor protein 4; cytochrome c oxidase subunit 6B; sperm protein associated with the nucleus on the X chromosome B; phospholipid hydroperoxide glutathione peroxidase-mitochondrial; isoaspartyl peptidase/L-asparaginase; heat shock-related 70 kDa protein 2; stress-70 protein, mitochondrial; glyceraldehyde-3-phosphate dehydrogenase, testis-specific and clusterin.

Conclusion

Fourteen proteins present in different amounts in asthenozoospermic sperm tail samples were identified, four of which are reported here for the first time. These proteins might be used as markers for the better diagnosis of sperm dysfunctions, targets for male contraceptive development, and to predict embryo quality.
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Metadata
Title
A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia
Authors
Mahmoud Hashemitabar
Susan Sabbagh
Mahmoud Orazizadeh
Atta Ghadiri
Maryam Bahmanzadeh
Publication date
01-06-2015
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 6/2015
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-015-0465-7

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