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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 4/2015

01-04-2015 | Fertility Preservation

Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?

Authors: Michelle Soares, Pascale Saussoy, Karima Sahrari, Christiani A. Amorim, Jacques Donnez, Marie-Madeleine Dolmans

Published in: Journal of Assisted Reproduction and Genetics | Issue 4/2015

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Abstract

Purpose

To evaluate the tumor-inducing ability of a few leukemic cells xenotransplanted inside an artificial ovary.

Methods

Ten and 100 BV-173 leukemic cells were embedded in a fibrin matrix along with 50,000 human ovarian stromal cells, and grafted to the peritoneal bursa of 5 and 5 SCID mice respectively. Four mice grafted with 3x106 leukemic cells in fibrin served as positive controls. At 20 weeks post-transplantation, the grafts, liver, spleen, blood and bone marrow were analyzed for the presence of leukemia by anti-CD79α IHC, flow cytometry (FC) and PCR.

Results

All mice grafted with 3x106 cells developed peritoneal masses 4 weeks after xenotransplantation, and systemic disease was confirmed by IHC, PCR and FC. Among mice grafted with 10 or 100 leukemic cells, none showed any sign of leukemia after 20 weeks, and IHC, FC and PCR on the different recovered tissues all proved negative.

Conclusion

This study investigates the tumor-inducing potential of a few leukemic cells grafted inside an artificial ovary. Transplantation of 100 leukemic cells appears to be insufficient to induce leukemia after 20 weeks. These results in an immunodeficient xenografting model are quite reassuring. However, for clinical application, follicle suspensions must be purged of leukemic cells before grafting, as even the slightest risk should be avoided.
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Metadata
Title
Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?
Authors
Michelle Soares
Pascale Saussoy
Karima Sahrari
Christiani A. Amorim
Jacques Donnez
Marie-Madeleine Dolmans
Publication date
01-04-2015
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 4/2015
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-015-0438-x

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