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Journal of Assisted Reproduction and Genetics

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01-10-2014 | Gonadal Physiology and Disease

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Authors: P. Piomboni, R. Focarelli, A. Capaldo, A. Stendardi, V. Cappelli, A. Cianci, A. La Marca, A. Luddi, V. De Leo

Published in: Journal of Assisted Reproduction and Genetics | Issue 10/2014

Abstract

Purpose

The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols.

Methods

FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free–SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria.

Results

Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free–SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P < 0.05) or metformin (P < 0.05) study groups in comparison to untreated control group.

Conclusion

A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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Title: Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin
Authors: P. Piomboni
R. Focarelli
A. Capaldo
A. Stendardi
V. Cappelli
A. Cianci
A. La Marca
A. Luddi
V. De Leo
Publication date: 01-10-2014
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 10/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0307-z

At a glance: The STEP trials

Springer Medicine

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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