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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 11/2013

01-11-2013 | Technological Innovations

In vitro development of human primordial follicles to preantral stage after vitrification

Authors: Fariba Khosravi, Robert L. Reid, Ashraf Moini, Farid Abolhassani, Mojtaba R. Valojerdi, Frederick W. K. Kan

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2013

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Abstract

Purpose

The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue.

Methods

Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed.

Results

High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P < 0.05) whereas primordial and transitory follicles showed a significant decrease (P < 0.05) compared to their respective counterparts at day 0 of culture.

Conclusions

Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.
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Metadata
Title
In vitro development of human primordial follicles to preantral stage after vitrification
Authors
Fariba Khosravi
Robert L. Reid
Ashraf Moini
Farid Abolhassani
Mojtaba R. Valojerdi
Frederick W. K. Kan
Publication date
01-11-2013
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 11/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-0105-z

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