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Journal of Assisted Reproduction and Genetics

Published in:

01-08-2013 | Assisted Reproduction Technologies

Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

Authors: Chloë Shaw-Jackson, Evelyne Bertrand, Bénédicte Becker, Jérôme Colin, Caroline Beaudoin-Chabot, Serge Rozenberg, Candice Autin

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2013

Abstract

Purpose

This study investigates whether certain embryos considered unsuitable for cryopreservation on day 3 might nevertheless have the potential to develop into worthwhile blastocysts that could be vitrified in the same cycle.

Methods

Retrospective study: between 2010 and 2011, embryo transfers and cryopreservation took place mainly on day 3 in our centre. Supernumerary embryos of intermediate to poor quality were reassessed on days 5/6 and any good quality blastocysts were vitrified.

Results

Out of 914 cleavage stage (day 3) embryos left in culture, 16 % were vitrified on days 5/6. Fifty blastocyst warming cycles resulted in a 76 % survival rate, 44 % clinical pregnancy rate and 39 % implantation rate. During the same time period, 213 warming cycles of good quality cleavage stage embryos rendered survival rates, clinical pregnancy and implantation rates of 97 %, 23 % and 16 % respectively.

Conclusions

Supernumerary average quality day 3 embryos should be given a second chance to be selected for cryopreservation. If blastocysts are obtained and survive vitrification, there is a good chance of implantation thus reducing embryo waste.

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Title: Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming
Authors: Chloë Shaw-Jackson
Evelyne Bertrand
Bénédicte Becker
Jérôme Colin
Caroline Beaudoin-Chabot
Serge Rozenberg
Candice Autin
Publication date: 01-08-2013
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 8/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0037-7

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Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

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