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Journal of Assisted Reproduction and Genetics

Published in:

01-10-2012 | ASSISTED REPRODUCTION TECHNOLOGIES

Day 5 expanded blastocysts transferred on same day have comparable outcome to those left for more extended culture and transferred on day 6

Authors: Eman Elgindy, Mervat Sheikh-El-Arab Elsedeek

Published in: Journal of Assisted Reproduction and Genetics | Issue 10/2012

Abstract

Objective

To study the outcome of blastocysts showing expansion on day 5 and transferred on day 5 or 6, in comparison with those unexpanded and transferred on day 6.

Study Design

Prospective cohort of 221 women prepared for BET classified into three groups according to timing of blastocyst expansion and day of embryo transfer. Group I; with expanded blastocysts on day 5 having day 5 transfer, group II; with expanded blastocysts on day 5 having day 6 transfer and group III ; with delayed expansion undergoing day 6 BET.

Results

Implantation rates, pregnancy rates, ongoing pregnancy rates, and live birth rates in the first 2 groups were almost double the rates in the third group. The figures for implantation rates were 40 % in the first two groups vs. 19 % in the third group (P < 0.05). Pregnancy rates were 60.9 % and 64 % vs. 31.8 % (P < 0.05) and ongoing pregnancy/ live-birth rates were 52.3 % & 56 % vs. 27.3 %.

Conclusion

The current study reports better implantation and pregnancy rates with earlier expanding blastocysts regardless of the time of transfer.

Papanikolaou EG, Kolibianakis EM, Tournaye H, et al. Live birth rates after transfer of equal number of blastocysts or cleavage-stage embryos in IVF. A systematic review and metaanalysis. Hum Reprod. 2008;23(1):91–9.PubMedCrossRef

Utsunomiya T, Ito H, Nagaki M, Sato J. A prospective, randomized study: day 3 versus hatching blastocyst stage. Hum Reprod. 2004;19:1598–603.PubMedCrossRef

Bungum M, Bungum L, Humaidan P, Yding Andersen C. Day 3 versus day 5 embryo transfer: a prospective randomized study. Reprod Biomed Online. 2003;7:98–104.PubMedCrossRef

Papanikolaou EG, Evangelos G, D’haeseleer E, et al. Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study. Hum Reprod. 2005;20:3198–203.PubMedCrossRef

Sills ES, Palermo GD. Human blastocyst culture in IVF: current laboratory applications in reproductive medicine practice. Rom J Morphol Embryol. 2010;51(3):441–5.PubMed

Huisman GJ, Fauser BC, Eijkemans MJ, Pieters MH. Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture. Fertil Steril. 2000;73:117–22.PubMedCrossRef

Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod. 1997;12:15459.CrossRef

Hsu MI, Mayer J, Aronshon M, et al. Embryo implantation in in vitro fertilization and intracytoplasmic sperm injection: impact of cleavage status, morphology grade, and number of embryos transferred. Fertil Steril. 1999;72:679–85.PubMedCrossRef

Shapiro BS, Richter KS, Harris DC, Daneshmand ST. A comparison of day 5 and day 6 blastocyst transfers. Fertil Steril. 2001;75:1126–30.PubMedCrossRef

10.

Shoukir Y, Chardonnens D, Campana A, Bischof P, Sakkas D. The rate of development and time of transfer play different roles in influencing the viability of human blastocysts. Hum Reprod. 1998;13:676–81.PubMedCrossRef

11.

Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Ross R. Contrasting patterns in in vitro fertilization pregnancy rates among fresh autologous, fresh oocyte donor and cryopreserved cycles with the use of day 5 or day 6 blastocysts may reflect differences in embryo-endometrium synchrony. Fertil Steril. 2008;89(1):20–6.PubMedCrossRef

12.

Barrenetxea G, de Larruzea A, Ganzabal T, Jiménez R, Carbonero K, Mandiola M. Blastocyst culture after repeated failure of cleavage stage embryo transfers: a comparison of day 5 and day 6 transfers. Fertil Steril. 2005;83:49–53.PubMedCrossRef

13.

Van Voorhis B, Dorkas A. Delayed blastocyst transfer: is the window shutting. Fertil Steril. 2008;89(1):31–2.PubMedCrossRef

14.

McArthur S, Don Leigh D, Marshal J, App B, de Boer K, Jansen R. Pregnancies and live births after trophectoderm biopsy and preimplantation genetic testing of human blastocysts. Fertil Steril. 2005;84:1628–36.PubMedCrossRef

15.

McArthur SJ, Leigh D, Marshall JT, Gee AJ, De Boer KA, Jansen RP. Blastocyst trophectoderm biopsy and preimplantation genetic diagnosis for familial monogenic disorders and chromosomal translocations. Prenat Diagn. 2008;28(5):434–42.PubMedCrossRef

16.

Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Thomas S. Large blastocyst diameter, early blastulation, and low preovulatory serum progesterone are dominant predictors of clinical pregnancy in fresh autologous cycles. Fertil Steril. 2008;90(2):302–9.PubMedCrossRef

17.

Veeck LL, Bodine R, Clarke RN, Berrios R, Libraro J, Moschini RM, et al. High pregnancy rates can be achieved after freezing and thawing human blastocysts. Fertil Steril. 2004;82:1418–27.PubMedCrossRef

18.

Behr B, Gebhardt J, Lyon J, Milki AA. Factors relating to successful cryopreserved blastocyst transfer program. Fertil Steril. 2002;77:697–9.PubMedCrossRef

19.

Van Landuyt L, Stoop D, Verheyen G, Verpoest W, Camus M, Van de Velde H, Devroey P, Van den Abbeel E. Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy. Hum Reprod. 2011;26(3):527–34.PubMedCrossRef

Title: Day 5 expanded blastocysts transferred on same day have comparable outcome to those left for more extended culture and transferred on day 6
Authors: Eman Elgindy
Mervat Sheikh-El-Arab Elsedeek
Publication date: 01-10-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 10/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9837-4

At a glance: The STEP trials

Springer Medicine

Day 5 expanded blastocysts transferred on same day have comparable outcome to those left for more extended culture and transferred on day 6

Abstract

Objective

Study Design

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Study Design

Results

Conclusion

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